Inhibition of CRIPTO expression and tumorigenicity in human colon cancer cells by antisense RNA and oligodeoxynucleotides (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Inhibition of CRIPTO expression and tumorigenicity in human colon cancer cells by antisense RNA and oligodeoxynucleotides (Articolo in rivista) (literal)

Anno

• 1994-01-01T00:00:00+01:00 (literal)

Alternative label

• Ciardiello, F., Tortora, G., Bianco, C., Selvam, M.P., Basolo, F., Fontanini, G., Pacifico, F., Normanno, N., Brandt, R., Persico, M.G., Salomon, D.S., Bianco, A.R. (1994)
  Inhibition of CRIPTO expression and tumorigenicity in human colon cancer cells by antisense RNA and oligodeoxynucleotides
  in Oncogene (Basingstoke)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Ciardiello, F., Tortora, G., Bianco, C., Selvam, M.P., Basolo, F., Fontanini, G., Pacifico, F., Normanno, N., Brandt, R., Persico, M.G., Salomon, D.S., Bianco, A.R. (literal)

Pagina inizio

• 291 (literal)

Pagina fine

• 298 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 9 (literal)

Rivista

• Oncogene (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 8 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 1 (literal)

Note

• PubMe (literal)
• ISI Web of Science (WOS) (literal)
• Scopus (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Cattedra di Oncologia Medica, Fac. di Medicina e Chirurgia, Univ. Studi di Napoli Federico II, 80131 Napoli, Italy; Div. Transfus. Sci., Food Drug A., Bethesda, MD 20892, United States; Ist. di Anat. e Istologia Patologica, Fac. di Medicina e Chirurgia, Univ. degli Studi di Pisa, 56100 Pisa, Italy; Ist. Internazionale Genet. e Biofis., CNR, 80125 Napoli, Italy; Lab. of Tumor Immunology and Biology, National Cancer Institute, National Institute of Health, Bethesda, MD 20892, United States (literal)

Titolo

• Inhibition of CRIPTO expression and tumorigenicity in human colon cancer cells by antisense RNA and oligodeoxynucleotides (literal)

Abstract

• CRIPTO is an epidermal growth factor-related gene expressed in a majority of human colorectal tumors. To assess the role of CRIPTO in the growth control of human colon cancer, we have treated human colon carcinoma GEO and CBS cells, that possess high levels of CRIPTO, and WIDR colon cancer cells, that are negative for CRIPTO expression, with two antisense phosphorothioate oligodeoxynucleotides complementary to the 5' end of the human CRIPTO mRNA. Both antisense oligodeoxynucleotides significantly reduced endogenous CRIPTO protein levels and inhibited GEO and CBS cell growth in monolayer and in semisolid medium, whereas they did not affect WIDR cell growth. In addition, GEO, CBS and WIDR cells were infected with a recombinant retroviral vector containing the hygromycin-resistance gene and a 900 bp EcoRI-EcoRI coding fragment of the human CRIPTO cDNA oriented in the 3' to 5' direction. GEO and CBS CRIPTO antisense infectants exhibited a 60 to 70% reduction in CRIPTO protein expression, in monolayer growth and in soft agar cloning efficiency as compared to parental noninfected cells. In contrast, infection of WIDR cells with the CRIPTO antisense retrovirus did not alter their growth. Finally, GEO CRIPTO antisense infectants formed tumors in nude mice that were significantly smaller and had a larger latency period as compared to noninfected GEO cells. (literal)

Prodotto di

• Istituto di genetica e biofisica \"Adriano Buzzati Traverso\" (IGB) (Istituto)
• Istituto per l'endocrinologia e l'oncologia \"Gaetano Salvatore\" (IEOS) (Istituto)

Autore CNR

• MARIA PERSICO (Persona)
• FRANCESCO MARIA PACIFICO (Persona)

Incoming links:

Autore CNR di

• FRANCESCO MARIA PACIFICO (Persona)
• MARIA PERSICO (Persona)

Prodotto

• Istituto per l'endocrinologia e l'oncologia \"Gaetano Salvatore\" (IEOS) (Istituto)
• Istituto di genetica e biofisica \"Adriano Buzzati Traverso\" (IGB) (Istituto)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Oncogene (Rivista)