Contributions of polar and apolar groups to the thermodynamic stability of Azurin (Articolo in rivista)

Type
Label
  • Contributions of polar and apolar groups to the thermodynamic stability of Azurin (Articolo in rivista) (literal)
Anno
  • 1996-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1007/BF02453268 (literal)
Alternative label
  • MILARDI D; S. FASONE; C. LA ROSA; D. GRASSO (1996)
    Contributions of polar and apolar groups to the thermodynamic stability of Azurin
    in Il Nuovo cimento della Società italiana di fisica. B (Testo stamp.)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • MILARDI D; S. FASONE; C. LA ROSA; D. GRASSO (literal)
Pagina inizio
  • 1347 (literal)
Pagina fine
  • 1354 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 18 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
  • 8 (literal)
Note
  • Scopus (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • MILARDI D; S. FASONE; C. LA ROSA; D. GRASSO, Dipartimento di Scienze Chimiche, Università degli Studi di Catania. (literal)
Titolo
  • Contributions of polar and apolar groups to the thermodynamic stability of Azurin (literal)
Abstract
  • All denaturational thermodynamic changes (?H, ?S, ?G) were calculated for the thermal unfolding of azurin from Pseudomonas Aeruginosa. DSC data could not be directly studied in terms of classical thermodynamics, because the thermal unfolding of azurin is an irreversible process. However, a mathematical extrapolation of heat capacity curves to infinite scan rate allowed us to obtain the denaturational enthalpy change and the melting temperature related to the reversible step. The denaturational heat capacity change (?Cp), together with all the relevant contributions of polar and apolar groups to the thermodynamic parameters of azurin were calculated according to an approach taking into account the common features of protein unfolding and dissolution of model compounds. A comparison between the experimental and the expected denaturational thermodynamic values was briefly discussed (literal)
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