Selection of reference genes in different myocardial regions of an in vivo ischemia/reperfusion rat model for normalization of antioxidant gene expression (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Selection of reference genes in different myocardial regions of an in vivo ischemia/reperfusion rat model for normalization of antioxidant gene expression (Articolo in rivista) (literal)

Anno

• 2012-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1186/1756-0500-5-124 (literal)

Alternative label

• Vesentini N, Barsanti C, Martino A, Kusmic C, Ripoli A, Rossi A, L'Abbate A (2012)
  Selection of reference genes in different myocardial regions of an in vivo ischemia/reperfusion rat model for normalization of antioxidant gene expression
  in BMC research notes
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Vesentini N, Barsanti C, Martino A, Kusmic C, Ripoli A, Rossi A, L'Abbate A (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 5 (literal)

Rivista

• BMC research notes (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 8 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 124 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• IFC-Istituto di Fisiologia Clinica, CNR, Pisa Scuola Superiore Sant'Anna, SSSA, Pisa Dipartimento di Biologia, Sezione Gentica, Università di Pisa (literal)

Titolo

• Selection of reference genes in different myocardial regions of an in vivo ischemia/reperfusion rat model for normalization of antioxidant gene expression (literal)

Abstract

• Background: Changes in cardiac gene expression due to myocardial injury are usually assessed in whole heart tissue. However, as the heart is a heterogeneous system, spatial and temporal heterogeneity is expected in gene expression. Results: In an ischemia/reperfusion (I/R) rat model we evaluated gene expression of mitochondrial and cytoplasmatic superoxide dismutase (MnSod, Cu-ZnSod) and thioredoxin reductase (trxr1) upon short (4 h) and long (72 h) reperfusion times in the right ventricle (RV), and in the ischemic/reperfused (IRR) and the remote region (RR) of the left ventricle. Gene expression was assessed by Real-time reverse-transcription quantitative PCR (RT-qPCR). In order to select most stable reference genes suitable for normalization purposes, in each myocardial region we tested nine putative reference genes by geNorm analysis. The genes investigated were: Actin beta (actb), Glyceraldehyde-3-P-dehydrogenase (gapdh), Ribosomal protein L13A (rpl13a), Tyrosine 3-monooxygenase (ywhaz), Beta-glucuronidase (gusb), Hypoxanthine guanine Phosphoribosyltransferase 1 (hprt), TATA binding box protein (tbp), Hydroxymethylbilane synthase (hmbs), Polyadenylate-binding protein 1 (papbn1). According to our findings, most stable reference genes in the RV and RR were hmbs/hprt and hmbs/tbp/hprt respectively. In the IRR, six reference genes were recommended for normalization purposes; however, in view of experimental feasibility limitations, target gene expression could be normalized against the three most stable reference genes (ywhaz/ pabp/hmbs) without loss of sensitivity. In all cases MnSod and Cu-ZnSod expression decreased upon long reperfusion, the former in all myocardial regions and the latter in IRR alone. trxr1 expression did not vary. Conclusions: This study provides a validation of reference genes in the RV and in the anterior and posterior wall of the LV of cardiac ischemia/reperfusion model and shows that gene expression should be assessed separately in each region. (literal)

Prodotto di

• Tassonomia clinica molecolare - Tassonomia clinica molecolare (ME.P05.003.001) (Modulo)
• Istituto di fisiologia clinica (IFC) (Istituto)

Autore CNR

• CLAUDIA KUSMIC (Unità di personale interno)
• NICOLETTA VESENTINI (Unità di personale interno)

Insieme di parole chiave

• Keywords of "Selection of reference genes in different myocardial regions of an in vivo ischemia/reperfusion rat model for normalization of antioxidant gene expression" (Insieme di parole chiave)

Incoming links:

Prodotto

• Istituto di fisiologia clinica (IFC) (Istituto)
• Tassonomia clinica molecolare - Tassonomia clinica molecolare (ME.P05.003.001) (Modulo)

Autore CNR di

• CLAUDIA KUSMIC (Unità di personale interno)
• NICOLETTA VESENTINI (Unità di personale interno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• BMC research notes (Rivista)

Insieme di parole chiave di

• Keywords of "Selection of reference genes in different myocardial regions of an in vivo ischemia/reperfusion rat model for normalization of antioxidant gene expression" (Insieme di parole chiave)