HCV type 2 infection show lower ALT levels and Higher HVR1 variation with respect to other viral types. (Abstract/Poster in atti di convegno)

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  • HCV type 2 infection show lower ALT levels and Higher HVR1 variation with respect to other viral types. (Abstract/Poster in atti di convegno) (literal)
Anno
  • 1996-01-01T00:00:00+01:00 (literal)
Alternative label
  • Brambilla S, Bellati G, Asti M, D'Amico M, Lisa A, Franchini A, Silini E. (1996)
    HCV type 2 infection show lower ALT levels and Higher HVR1 variation with respect to other viral types.
    in IX Triennial International Symposium on Viral Hepatitis and Liver Disease, Roma, 21-25 Aprile 1996
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Brambilla S, Bellati G, Asti M, D'Amico M, Lisa A, Franchini A, Silini E. (literal)
Pagina inizio
  • 28 (literal)
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  • Proceedings IX Triennial International Symposium on Viral Hepatitis and Liver Disease (literal)
Note
  • Poster (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Department of Pathology, 1RCCS S.Matteo and University, Pavia; Division of Hepitology, Niguarda Hospital, Milano; Population Genetics Lab.; IGM-CNR Pavia. (literal)
Titolo
  • HCV type 2 infection show lower ALT levels and Higher HVR1 variation with respect to other viral types. (literal)
Abstract
  • Objective: To determine hepatitis C vims (HCV) quasi species (QS) in the hypervariable region 1 (HVRIJ region of ihe E2 gene and 10 correal? QS structure and evolution with genotypes, serum RNA titers and ALT levels. Methods: 53 consecutive anti-HCV+ patients with chronic hepatitis were studied, all were new referrals, followed up for al least one year with monthly ALT determinations; 25 of them were considered for analysis of QS. HCV genotype was determined according to Okamoto. HCV RNA liters were assayed by competitive PCR at entry* (t0) and after 3, 6, 9 and 12 months (t12). HVRI sequences were PCR-amplified from sera at tO and t12, 10 clones were sequenced at each lime point. QS evolution was determined by construction of pay-logenetic trees using the neighbour joining method and evolutionary distances were calculated according to Kimura. Result: HCV type distribution in our cohort of 53 patients was as follows; HCV type la 2 patients, type lb 27, type 2a 16, type 3a 5 and uniypable 3 patients. Mean ALT level were-correiated with the HCV type, being significanlty lower in type 2 infected patients (75±52UI) than in other types (125±80, p<0.02 by analysis of variance). No relationships were found between the number of HVRI variants present at a single point and the infecting genotypes, the ALT levels or the RNA liters. Furthermore, QS complexity in individual patients could either increase or decrease over time in an unpredictable way. Conversely, relevant differences in HVRI sequence variation during the period of follow-up were observed, in particular 6 patients showed no change in QS and they were all infected by HCV type 1. Mean evolutionary distances between 10 and 112 HVRI variants in each patient, as assessed by calculation of Kimura distances, were significantly different according to the infecting genotype, being significantly higher for patients with type 2a infection (0.260+/-0.38) with respect to other types (0.071 ±0.01, p< O.001 by t test). Conclusions: HCV type 2 infection is associated with lower ALT levels as compared with other viral types. HVR1 variation over time in type 2 infected patients is significantly higher than in other HCV types, indicating a more active immune selection in this region. These observations suggest important clues to the understanding of the pathogenetic diffemnces among HCV types and the mechanisms underlying HCV persistence and fiver damage. (literal)
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