AGO/RISC-mediated antiviral RNA silencing in a plant in vitro system (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• AGO/RISC-mediated antiviral RNA silencing in a plant in vitro system (Articolo in rivista) (literal)

Anno

• 2013-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1093/nar/gkt193 (literal)

Alternative label

• Schuck J., Gursinsky T., Pantaleo V., Burgyan J., Behrens S.E. (2013)
  AGO/RISC-mediated antiviral RNA silencing in a plant in vitro system
  in Nucleic acids research (Online); Oxford University Press, Oxford (Regno Unito)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Schuck J., Gursinsky T., Pantaleo V., Burgyan J., Behrens S.E. (literal)

Pagina inizio

• 5090 (literal)

Pagina fine

• 5103 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 41 (literal)

Rivista

• Nucleic acids research (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 14 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 9 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• SJ, GT, BSE: Institute of Biochemistry and Biotechnology (NFI), Martin Luther University Halle-Wittenberg, Germany. PV: Istituto di Virologia Vegetale (IVV) del CNR, Research Unit of Bari, Italy BJ: Agricultural Biotechnology Center, Ministry of Rural Development, Gödöllö, Hungary (literal)

Titolo

• AGO/RISC-mediated antiviral RNA silencing in a plant in vitro system (literal)

Abstract

• AGO/RISC-mediated antiviral RNA silencing, an important component of the plant's immune response against RNA virus infections, was recapitulated in vitro. Cytoplasmic extracts of tobacco protoplasts were applied that supported Tombusvirus RNA replication, as well as the formation of RNA-induced silencing complexes (RISC) that could be functionally reconstituted with various plant ARGONAUTE (AGO) proteins. For example, when RISC containing AGO1, 2, 3 or 5 were programmed with exogenous siRNAs that specifically targeted the viral RNA, endonucleolytic cleavages occurred and viral replication was inhibited. Antiviral RNA silencing was disabled by the viral silencing suppressor p19 when this was present early during RISC formation. Notably, with replicating viral RNA, only (+)RNA molecules were accessible to RISC, whereas (-)RNA replication intermediates were not. The vulnerability of viral RNAs to RISC activity also depended on the RNA structure of the target sequence. This was most evident when we characterized viral siRNAs (vsiRNAs) that were particularly effective in silencing with AGO1- or AGO2/RISC. These vsiRNAs targeted similar sites, suggesting that accessible parts of the viral (+)RNA may be collectively attacked by different AGO/RISC. The in vitro system was, hence, established as a valuable tool to define and characterize individual molecular determinants of antiviral RNA silencing. (literal)

Editore

• Oxford University Press (Editore)

Prodotto di

• Interazioni biologiche e molecolari delle piante con virus e agenti patogeni virus-simili (AG.P01.010.001) (Modulo)

Autore CNR

• VITANTONIO PANTALEO (Persona)

Insieme di parole chiave

• Keywords of "AGO/RISC-mediated antiviral RNA silencing in a plant in vitro system" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• VITANTONIO PANTALEO (Persona)

Prodotto

• Interazioni biologiche e molecolari delle piante con virus e agenti patogeni virus-simili (AG.P01.010.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Nucleic acids research (Rivista)

Editore di

• Oxford University Press (Editore)

Insieme di parole chiave di

• Keywords of "AGO/RISC-mediated antiviral RNA silencing in a plant in vitro system" (Insieme di parole chiave)