SPR based immunosensor for detection of Legionella pneumophila in water samples (Articolo in rivista)

Type
Label
  • SPR based immunosensor for detection of Legionella pneumophila in water samples (Articolo in rivista) (literal)
Anno
  • 2013-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1016/j.optcom.2012.12.064i (literal)
Alternative label
  • De Lorenzis E, M.G. Manera, G. Montagna, F. Cimaglia, M. Chiesa, P. Poltronieri , A. Santino, Rella R. (2013)
    SPR based immunosensor for detection of Legionella pneumophila in water samples
    in Optics communications (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • De Lorenzis E, M.G. Manera, G. Montagna, F. Cimaglia, M. Chiesa, P. Poltronieri , A. Santino, Rella R. (literal)
Pagina inizio
  • 420 (literal)
Pagina fine
  • 426 (literal)
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  • Impact Factor 1.542 (literal)
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  • http://dx.doi.org/10.1016/j.optcom.2012.12.064i (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 294 (literal)
Rivista
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  • 7 (literal)
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  • a Department of Biological and Environmental Sciences and Technologies, Biotecgen srl., University of Salento, Via Provinciale le Lecce-Monteroni, Lecce, Italy b IMM-CNR Institute for Microelectronic and Microsystems, Unit of Lecce, Campus Ecotekne, Via Monteroni, 73100 Lecce, Italy c ISPA, Institute of Sciences of Food Production C.N.R., Unit of Lecce, Via Monteroni, Lecce 73100, Italy (literal)
Titolo
  • SPR based immunosensor for detection of Legionella pneumophila in water samples (literal)
Abstract
  • Detection of legionellae by water sampling is an important factor in epidemiological investigations of Legionnaires' disease and its prevention. To avoid labor-intensive problems with conventional methods, an alternative, highly sensitive and simple method is proposed for detecting L. pneumophila in aqueous samples. A compact Surface Plasmon Resonance (SPR) instrumentation prototype, provided with proper microfluidics tools, is built. The developed immunosensor is capable of dynamically following the binding between antigens and the corresponding antibody molecules immobilized on the SPR sensor surface. A proper immobilization strategy is used in this work that makes use of an important efficient step aimed at the orientation of antibodies onto the sensor surface. The feasibility of the integration of SPR-based biosensing setups with microfluidic technologies, resulting in a low-cost and portable biosensor is demonstrated. (literal)
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