http://www.cnr.it/ontology/cnr/individuo/prodotto/ID210268

Cytotoxicity induced by exposure to natural and synthetic tremolite asbestos: An in vitro pilot study. (Articolo in rivista)

Type

Prodotto della ricerca (Classe)
Articolo in rivista (Classe)

Label

Cytotoxicity induced by exposure to natural and synthetic tremolite asbestos: An in vitro pilot study. (Articolo in rivista) (literal)

Anno

2013-01-01T00:00:00+01:00 (literal)

Alternative label

Pugnaloni A. [1]; Giantomassi F. [1]; Lucarini G. [1]; Capella S. [2, 5] Bloise A. [3]; Di Primio R. [1]; Belluso E. [2, 4, 5] (2013)
Cytotoxicity induced by exposure to natural and synthetic tremolite asbestos: An in vitro pilot study.
in Acta histochemica (Print); Elsevier, Oxford (Regno Unito)
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Pugnaloni A. [1]; Giantomassi F. [1]; Lucarini G. [1]; Capella S. [2, 5] Bloise A. [3]; Di Primio R. [1]; Belluso E. [2, 4, 5] (literal)

Pagina inizio

100 (literal)

Pagina fine

112 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

http://www.elsevier.com/journals/acta-histochemica/0065-1281 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

115 (literal)

Rivista

Acta histochemica (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

2 (literal)

Note

ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

[1] Dipartimento di Scienze Cliniche e Molecolari-Istologia, Università Politecnica delle Marche, Ancona, Italy [2] Dipartimento di Scienze della Terra, Università degli Studi di Torino, Torino, Italy [3]Dipartimento di Scienze della Terra, Università degli Studi della Calabria, Cosenza, Italy [4] Istituto di Geoscienze e Georisorse, CNR-Unita' di Torino, Torino, Italy [5] Centro Interdipartimentale per lo Studio degli Amianti e di altri Particolati Nocivi \"Giovanni Scansetti\", Università degli Studi di Torino, Torino, Italy (literal)

Titolo

Cytotoxicity induced by exposure to natural and synthetic tremolite asbestos: An in vitro pilot study. (literal)

Abstract

Mineral fibers are potential carcinogens to humans. In order to help clarify the etiology of the pathological effects of asbestos, cellular reactions to natural and synthetic asbestos fibers were compared using a lung alveolar cancer cell line (A549 epithelial cells), considered the first target of inhaled micro-environmental contaminants. Natural asbestos tremolite (NAT) fibers were collected from rocks in NW Italy. Synthetic asbestos tremolite (SAT) was iron-free and therefore considered as standard tremolite. Both fibers, subjected to mineralogical characterization by X-ray powder diffractometry, electron microscopy and energy dispersive spectrometry, fell within the definition of respirable and potentially carcinogenic fibers. Several signs of functional and structural cell damage were found after treatment with both fibers, documented by viability, motility, and morphological perturbations. Phalloidin labeling showed irregular distribution of cytoskeletal F-actin, whereas immunohistochemical investigations showed abnormal expression of VEGF, Cdc42, ?-catenin, assessed as risks indicators for cancer development. Both fibers caused significant loss of viability, even compared to UICC crocidolite, but, while SAT fibers exerted a more direct cytotoxic effect, survival of damaged cells expressing high VEGF levels was detected after NAT contact. This in vitro pilot study outlines potential health risks of NAT fibers in vivo related to their iron content, which could trigger signaling networks connected with cell proliferation and neoplastic transformation. (literal)

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