http://www.cnr.it/ontology/cnr/individuo/prodotto/ID20955
Aurora B expression directly correlates with prostate cancer malignancy and influence prostate cell proliferation (Articolo in rivista)
- Type
- Label
- Aurora B expression directly correlates with prostate cancer malignancy and influence prostate cell proliferation (Articolo in rivista) (literal)
- Anno
- 2006-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1002/pros.20345 (literal)
- Alternative label
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Chieffi P; Cozzolino L; Kisslinger A; Libertini S; Staibano S; Mansueto G; De Rosa G; Villacci A; Vitale M; Linardopoulos S; Portella G: Tramontano D. (literal)
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- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Dipartimento di Medicina Sperimentale, II Università di Napoli, Naples, Italy
Dipartimento di Scienze Biomorfologiche e Funzionali, Università di Napoli \"Federico II,\" Naples, Italy
IEOS/CNR, Naples, Italy
Dipartimento di Biologia e Patologia Cellulare e Molecolare, Università di Napoli \"Federico II,\" Naples, Italy
Dipartimento di Endocrinologia e Oncologia Molecolare e Clinica, Università di Napoli \"Federico II,\" Naples, Italy
The Breakthrough Breast Cancer Research Centre, London, United Kingdom (literal)
- Titolo
- Aurora B expression directly correlates with prostate cancer malignancy and influence prostate cell proliferation (literal)
- Abstract
- BACKGROUND.Chromosomal instability is one of the most common features of prostate cancer (PC), especially in advanced stages. Recent studies suggest that defects in mitotic checkpoints play a role in carcinogenesis. Lack of mitotic regulation induces aneuploidy in cancer cells acting thereafter as a driving force for malignant progression. Serine/threonine proteinkinases of theAurora genes familyplay an important throughout the entire cell cycle. In that Aurora B regulates chromosome segregation by ensuring the orientation of sister chromatids. As a consequence, the overexpression of Aurora B in diploid human cells NHDF induces the appearance of multinucleate cells. METHODS.Archive samples of normal and neoplastic prostate tissue, and prostate derived cell lines were screened for the expression of Aurora B. RESULTS. Immunohistochemical analysis showed increased nuclear expression of Aurora-B in high Gleason grade PCs respect to low and intermediate grade cases and in all cancers in respect to hyperplastic and normal glands. Furthermore, in the high Gleason grade anaplastic cancer tissuesAuroraB expression was accompaniedby the phosphorylationof the histoneH3. In analogy to the in vivo situation, Aurora B was vigorously expressed in the androgen independent PC cell lines PC3 and DU145, while a very modest expression of the kinase was observed in the androgen sensitive LnCap cells and in the EPN cells, a line of epithelial cells derived from normal prostate tissue. In addition, in PC3 cells Aurora B expression is ac-companied the by the phosphorylation of the histone H3. The block of Aurora B expression induced by an inhibitor of Aurora kinase activity significantly reduced the growth of prostate carcinoma cells, but not that of non-transformed EPN cells. (literal)
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