Evidence of Bacteroides fragilis protection from Bartonella henselae-induced damage. (Articolo in rivista)

Type
Label
  • Evidence of Bacteroides fragilis protection from Bartonella henselae-induced damage. (Articolo in rivista) (literal)
Anno
  • 2013-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1371/journal.pone.0049653 (literal)
Alternative label
  • Sommese L, Pagliuca C, Avallone B, Ippolito R, Casamassimi A, Costa V, Colicchio R, Cerciello R, D'Armiento M, Scarpato M, Giovane A, Pastore G, Infante T, Ciccodicola A, Fiorito C, D'Armiento FP, Salvatore P, Napoli C. (2013)
    Evidence of Bacteroides fragilis protection from Bartonella henselae-induced damage.
    in PloS one
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Sommese L, Pagliuca C, Avallone B, Ippolito R, Casamassimi A, Costa V, Colicchio R, Cerciello R, D'Armiento M, Scarpato M, Giovane A, Pastore G, Infante T, Ciccodicola A, Fiorito C, D'Armiento FP, Salvatore P, Napoli C. (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 7 (11 (literal)
Rivista
Note
  • PubMe (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Division of Immunohematology, Transfusion Medicine and Transplant Immunology, Regional Reference Laboratory of Transplant Immunology, Azienda Universitaria Policlinico, 1st School of Medicine, Second University of Naples, Naples, Italy Section of Microbiology, Department of Experimental Medicine, 1st School of Medicine Second University of Naples, Naples, Italy, 3 CEINGE-Advanced Biotechnologies, Naples, Italy, Department of Biological Science, University of Naples ''Federico II'', Naples, Italy Department of Biomorphological and Functional Science, University of Naples ''Federico II'', Naples, Italy Department of General Pathology and Excellence Research Centre on Cardiovascular Diseases, 1st School of Medicine, Second University of Naples, Naples, Italy CNR, Institute of Genetics and Biophysics ''A. Buzzati-Traverso'', Naples, Italy, Foundation-SDN, Institute of Diagnostic and Nuclear Development, IRCCS, Naples, Italy Department of Biochemistry and Biophysics, Second University of Naples, Naples, Italy Department of Sciences for Biology, Geology, and Environment, University of Sannio, Benevento, Italy Department of Cellular and Molecular Biology and Pathology ''L. Califano'', University of Naples ''Federico II'', Naples, Italy (literal)
Titolo
  • Evidence of Bacteroides fragilis protection from Bartonella henselae-induced damage. (literal)
Abstract
  • Bartonella henselae is able to internalize endothelial progenitor cells (EPCs), which are resistant to the infection of other common pathogens. Bacteroides fragilis is a gram-negative anaerobe belonging to the gut microflora. It protects from experimental colitis induced by Helicobacter hepaticus through the polysaccharide A (PSA). The aim of our study was to establish: 1) whether B. fragilis colonization could protect from B. henselae infection; if this event may have beneficial effects on EPCs, vascular system and tissues. Our in vitro results establish for the first time that B. fragilis can internalize EPCs and competes with B. henselae during coinfection. We observed a marked activation of the inflammatory response by Real-time PCR and ELISA in coinfected cells compared to B. henselae-infected cells (63 vs 23 up-regulated genes), and after EPCs infection with mutant B. fragilis ?PSA (?90% up-regulated genes) compared to B. fragilis. Interestingly, in a mouse model of coinfection, morphological and ultrastructural analyses by hematoxylin-eosin staining and electron microscopy on murine tissues revealed that damages induced by B. henselae can be prevented in the coinfection with B. fragilis but not with its mutant B. fragilis ?PSA. Moreover, immunohistochemistry analysis with anti-Bartonella showed that the number of positive cells per field decreased of at least 50% in the liver (20±4 vs 50±8), aorta (5±1 vs 10±2) and spleen (25±3 vs 40±6) sections of mice coinfected compared to mice infected only with B. henselae. This decrease was less evident in the coinfection with ?PSA strain (35±6 in the liver, 5±1 in the aorta and 30±5 in the spleen). Finally, B. fragilis colonization was also able to restore the EPC decrease observed in mice infected with B. henselae (0.65 vs 0.06 media). Thus, our data establish that B. fragilis colonization is able to prevent B. henselae damages through PSA. (literal)
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