Soil Enzyme assays using P-nitrophenil derivates: an Inter-laboratory comparison (Abstract/Poster in convegno)

Type
Label
  • Soil Enzyme assays using P-nitrophenil derivates: an Inter-laboratory comparison (Abstract/Poster in convegno) (literal)
Anno
  • 2011-01-01T00:00:00+01:00 (literal)
Alternative label
  • Luigi Balducco; Vito Armando Laudicina; Flavio Fornasier; Emauele Vida; Sara Marinari; Maria Cristina Moscatelli; Grazia Masciandaro; Brunello Ceccanti; Maria Antonietta Rao; Rosalia Scelza (2011)
    Soil Enzyme assays using P-nitrophenil derivates: an Inter-laboratory comparison
    in ENZYMES: Enzymes in the Environment: Activity, Ecology, & Applications, Bad Nauheim, Germania, 17-21 luglio 2011
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Luigi Balducco; Vito Armando Laudicina; Flavio Fornasier; Emauele Vida; Sara Marinari; Maria Cristina Moscatelli; Grazia Masciandaro; Brunello Ceccanti; Maria Antonietta Rao; Rosalia Scelza (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni
  • ID_PUMA:/cnr.ise/2011-A3-026Area di valutazione 04 - Scienze della Terra (literal)
Note
  • Poster (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Luigi Balducco; Vito Armando Laudicina: Dipartimento di Ingegneria e Tecnologie Agro-Forestali, Università di Palermo; Flavio Fornasier; Emauele Vida: CRA-RPS Centro di Ricerca per lo Studio delle Relazioni tra Pianta e Suolo, sede di Gorizia; Sara Marinari; Maria Cristina Moscatelli: Università della Tuscia - Viterbo; Maria Antonietta Rao; Rosalia Scelza: Dipartimento di Scienza del Suolo, della Pianta, dell'Ambiente e delle Produzioni Animali, Università Federico II di Napoli, Portici (NA) (literal)
Titolo
  • Soil Enzyme assays using P-nitrophenil derivates: an Inter-laboratory comparison (literal)
Abstract
  • Reproducibility and reliability of soil enzyme tests needs to be validated by comparing results among labs. The Italian Group of Enzymology organized an inter-laboratory comparison: five labs participated. Beta-glucosidase- acid- and alkaline phosphatase were chosen as enzyme activities to be determined: p-nitrophenyl derivatives, due to wide utilization, were chosen as substrates. Procedure for each enzymatic activity was discussed, considering practical and available factors such as available instruments. Nine soils with contrasting physico-chemical properties were carefully dried and sieved to ensure homogeneity. They were sent to each laboratory. Results of tests were evaluated using the z-score. Systematic error and random error of a specific lab were calculated as the average z-score and standard deviation of z-score for a given enzyme in that lab. Due to large correlation between the parameter and the error term, only z-scores below 1 were considered as satisfactory Two labs had acceptable systematic errors for all three enzymes, i.e. average z-score always less than 1. Although the number of labs participating was small, some indications emerged: i- systematic error appear, to be enzyme-dependent; each lab tends to over- or underestimate any enzymatic activity; ii- random errors were enzyme-dependent, some labs have random errors lower than others; iii-beta-glucosidase is the enzyme which measurement is, in general, less variable among labs. This inter-laboratory comparison improved awareness of soil enzyme tests reliability performed in their lab; it is the start to identify critical steps for more reliable protocols. (literal)
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