The Sso7d DNA-binding protein from Sulfolobus solfataricus has ribonuclease activity (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• The Sso7d DNA-binding protein from Sulfolobus solfataricus has ribonuclease activity (Articolo in rivista) (literal)

Anno

• 2001-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/S0014-5793(01)02455-3 (literal)

Alternative label

• Shehi, E; Serina, S; Fumagalli, G; Vanoni, M; Consonni, R; Zetta, L; Deho, G; Tortora, P; Fusi, P (2001)
  The Sso7d DNA-binding protein from Sulfolobus solfataricus has ribonuclease activity
  in FEBS letters (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Shehi, E; Serina, S; Fumagalli, G; Vanoni, M; Consonni, R; Zetta, L; Deho, G; Tortora, P; Fusi, P (literal)

Pagina inizio

• 131 (literal)

Pagina fine

• 136 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

• http://www.sciencedirect.com/science/article/pii/S0039914007002366 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 497 (literal)

Rivista

• FEBS letters (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Consonni, R; Zetta, L ISMAC Milano (literal)

Titolo

• The Sso7d DNA-binding protein from Sulfolobus solfataricus has ribonuclease activity (literal)

Abstract

• Sso7d is a small, basic, abundant protein from the thermoacidophilic archaeon Sulfolobus solfataricus. Previous research has shown that Sso7d can bind double-stranded DNA without sequence specificity by placing its triple-stranded P-sheet across the minor groove. We previously found RNase activity both in preparations of Sso7d purified from its natural source and in recombinant, purified protein expressed in Escherichia coli, This paper provides conclusive evidence that supports the assignment of RNase activity to Sso7d, shown by the total absence of activity in the single-point mutants E35L and K12L, despite the preservation of their overall structure under the assay conditions. In keeping with our observation that the residues putatively involved in RNase activity and those playing a role in DNA binding are located on different surfaces of the molecule, the activity was not impaired in the presence of DNA, If a small synthetic RNA was used as a substrate, Sso7d attacked both predicted double- and single-stranded RNA stretches, with no evident preference for specific sequences or individual bases. Apparently, the more readily attacked bonds were those intrinsically more unstable. (C) 2001 Federation of European Biochemical Societies. (literal)

Prodotto di

• Studio di metaboliti e matrici naturali mediante NMR e loro utilizzo e funzione nel settore agroalimentare, merceologico e della salute umana (PM.P06.018.001) (Modulo)
• Istituto per lo studio delle macromolecole (ISMAC) (Istituto)

Autore CNR

• ROBERTO CONSONNI (Unità di personale interno)

Insieme di parole chiave

• Keywords of "The Sso7d DNA-binding protein from Sulfolobus solfataricus has ribonuclease activity" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• ROBERTO CONSONNI (Unità di personale interno)

Prodotto

• Istituto per lo studio delle macromolecole (ISMAC) (Istituto)
• Studio di metaboliti e matrici naturali mediante NMR e loro utilizzo e funzione nel settore agroalimentare, merceologico e della salute umana (PM.P06.018.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• FEBS letters (Rivista)

Insieme di parole chiave di

• Keywords of "The Sso7d DNA-binding protein from Sulfolobus solfataricus has ribonuclease activity" (Insieme di parole chiave)