Simultaneous speciation analysis of glutathione peroxidase, selenoprotein P and selenoalbumin in human serum by tandem anion exchange-affinity HPLC and on-line isotope dilution ICP-quadrupole MS (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Simultaneous speciation analysis of glutathione peroxidase, selenoprotein P and selenoalbumin in human serum by tandem anion exchange-affinity HPLC and on-line isotope dilution ICP-quadrupole MS (Articolo in rivista) (literal)

Anno

• 2008-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1007/s00216-008-2043-7 (literal)

Alternative label

• Jitaru P., Cozzi G., Gambaro A., Cescon P., Barbante C. (2008)
  Simultaneous speciation analysis of glutathione peroxidase, selenoprotein P and selenoalbumin in human serum by tandem anion exchange-affinity HPLC and on-line isotope dilution ICP-quadrupole MS
  in Analytical & bioanalytical chemistry (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Jitaru P., Cozzi G., Gambaro A., Cescon P., Barbante C. (literal)

Pagina inizio

• 661 (literal)

Pagina fine

• 669 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 391 (literal)

Rivista

• Analytical & bioanalytical chemistry (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 9 (literal)

Note

• Google Scholar (literal)
• ISI Web of Science (WOS) (literal)
• Scopus (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Institute for the Dynamics of Environmental Processes (IDPA-CNR), Dorsoduro 2137, 30123 Venice, Italy Department of Inorganic and Analytical Chemistry, Faculty of Chemistry, University \"Al. I. Cuza\", 11 Carol I Blvd., 700506 Iasi, Romania Department of Environmental Sciences, University of Venice Ca' Foscari, Dorsoduro 2137, 30123 Venice, Italy e-mail: barbante@unive.it (literal)

Titolo

• Simultaneous speciation analysis of glutathione peroxidase, selenoprotein P and selenoalbumin in human serum by tandem anion exchange-affinity HPLC and on-line isotope dilution ICP-quadrupole MS (literal)

Abstract

• A method based on anion exchange (AE) and affinity (AF)-HPLC(AE-AF- HPLC) hyphenated to inductively coupled plasma-(quadrupole) mass spectrometry (ICP- QMS) was developed for the speciation analysis of selenoprotein P (SelP), glutathione peroxidase (GPx) and selenoalbumin (SeAlb) in human serum. AE- HPLC is proposed here for the on- line alleviation of Cl and Br spectral interferences on Se-77 ((ArCl)-Ar-40-Cl-37) and Se-82 ((BrH)-Br-81-H-1). Separation of GPx, SelP and SeAlb by AE-AF- HPLC was obtained within a total chromatographic runtime of < 20 min. On-line (post-column) isotope dilution (ON-ID) and on- line external calibration (ON-EC)ICP- QMS were used for the quantification of Se in GPx, SelP and SeAlb. ON-EC using a Se-L-cystine standard was shown to be a suitable approach for the routine simultaneous speciation analysis of serum GPx, SelP and SeAlb. The method validation was carried out by direct ICP-sector field MS determination of Se in GPx, SelP and SeAlb fractions collected after AE-AF-HPLC separation. In addition, the method accuracy for the determination of total protein-bound Se was assessed by analyzing a human serum reference material (BCR-637) certified for total Se content. (literal)

Prodotto di

• Microinquinanti organici ed inorganici in aree a diversa antropizzazione (TA.P03.011.001) (Modulo)
• Istituto per la dinamica dei processi ambientali (IDPA) (Istituto)

Autore CNR

• PETRU JITARU (Unità di personale esterno)
• PAOLO CESCON (Unità di personale esterno)
• CARLO BARBANTE (Unità di personale interno)
• GIULIO COZZI (Unità di personale interno)
• ANDREA GAMBARO (Persona)

Insieme di parole chiave

• Keywords of "Simultaneous speciation analysis of glutathione peroxidase, selenoprotein P and selenoalbumin in human serum by tandem anion exchange-affinity HPLC and on-line isotope dilution ICP-quadrupole MS" (Insieme di parole chiave)

Incoming links:

Prodotto

• Istituto per la dinamica dei processi ambientali (IDPA) (Istituto)
• Microinquinanti organici ed inorganici in aree a diversa antropizzazione (TA.P03.011.001) (Modulo)

Autore CNR di

• CARLO BARBANTE (Unità di personale interno)
• PETRU JITARU (Unità di personale esterno)
• PAOLO CESCON (Unità di personale esterno)
• ANDREA GAMBARO (Persona)
• GIULIO COZZI (Unità di personale interno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Analytical & bioanalytical chemistry (Rivista)

Insieme di parole chiave di

• Keywords of "Simultaneous speciation analysis of glutathione peroxidase, selenoprotein P and selenoalbumin in human serum by tandem anion exchange-affinity HPLC and on-line isotope dilution ICP-quadrupole MS" (Insieme di parole chiave)