Expression patterns of Pl-c-jun during Paracentrotus lividus sea urchin development. (Abstract/Poster in atti di convegno)

Type
Label
  • Expression patterns of Pl-c-jun during Paracentrotus lividus sea urchin development. (Abstract/Poster in atti di convegno) (literal)
Anno
  • 2012-01-01T00:00:00+01:00 (literal)
Alternative label
  • Russo R., Zito F., Pinsino A., Bonaventura R.and Matranga V. (2012)
    Expression patterns of Pl-c-jun during Paracentrotus lividus sea urchin development.
    in FISV 2012, Roma, 24-27/9/12
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Russo R., Zito F., Pinsino A., Bonaventura R.and Matranga V. (literal)
Pagina inizio
  • 66 (literal)
Pagina fine
  • 66 (literal)
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  • Book of abstract FISV 2012 (literal)
Note
  • Abstract (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Istituto di Biomedicina e Immunologia Molecolare ''Alberto Monroy'', Consiglio Nazionale delle Ricerche, Via Ugo La Malfa 153, 90146 Palermo, ITALY (literal)
Titolo
  • Expression patterns of Pl-c-jun during Paracentrotus lividus sea urchin development. (literal)
Abstract
  • Abstract The AP-1 transcription factor is involved in many cellular events such as cell cycle, cell proliferation, differentiation. It consists of dimers composed of different proteins, c-jun, junB, c-fos, Fra-1 and Fra-2. We isolated the complete cDNA encoding the Pl-c-jun gene from P.lividus embryos and described the phylogenetic relationships with homologs of different phyla. We found that Pl-c-jun mRNA levels, measured by QPCR, were high in unfertilized egg, and decreased during development if compared to egg's mRNA. The spatial expression of Pl-c-jun mRNA, detected by WMISH in embryos at different developmental stages, showed ubiquitous transcripts in the cleavage stages. Then, Pl-c-jun mRNA was restricted in a few cells at the vegetative region of blastula and in some skeletogenic cells, the Primary Mesenchyme Cells (PMC), in gastrula and pluteus embryos. The localization of c-jun protein was studied by immunofluorescence experiments on whole mount embryos using commercial antibodies. The presence of total c-jun protein in the PMCs was confirmed, showing a cytoplasmic localization, while its phosphorylated form was restricted to the nucleus of the same cells. Preliminary Western Blot of total c-jun protein, in subcellular fractionation experiments, showed nuclear and cytoplasmic localization too. The specific localization of Pl-c-jun in PMC suggests a role as a regulatory element of skeletogenic genes. (literal)
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