Rat bone regeneration induced by the engraftment of human amniotic fluid stem cells (AFSC) unselected for c-Kit: a pilot study (Contributo in atti di convegno)

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  • Rat bone regeneration induced by the engraftment of human amniotic fluid stem cells (AFSC) unselected for c-Kit: a pilot study (Contributo in atti di convegno) (literal)
Anno
  • 2012-01-01T00:00:00+01:00 (literal)
Alternative label
  • Centurione M A 1, Centurione L 2 3, Marchegiani F 2, Pantalone A 3 4, I. Antonucci 3 5, Vanni D 4, Sancilio S 2 3, Salini V 4, Stuppia L 3 5, Di Pietro R 2 3 (2012)
    Rat bone regeneration induced by the engraftment of human amniotic fluid stem cells (AFSC) unselected for c-Kit: a pilot study
    in ABCD: stem cells, development and regenerative medicine, Turin, 4-6 maggio 2012
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Centurione M A 1, Centurione L 2 3, Marchegiani F 2, Pantalone A 3 4, I. Antonucci 3 5, Vanni D 4, Sancilio S 2 3, Salini V 4, Stuppia L 3 5, Di Pietro R 2 3 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • 1Inst. Molecular Genetics, National Research Council, Chieti, Italy 2Dept. Medicine and Ageing Sciences, G. d'Annunzio University, Chieti-Pescara, Italy 3Aging Research Center, Ce.S.I., G. d'Annunzio University Foundation, StemTeCh Group, Chieti, Italy 4Orthopaedic and Traumatologic Division, G. d'Annunzio University, Chieti-Pescara, Italy 5Dept. Oral Sciences, Nano and Biotechnologies, G. d'Annunzio University, Chieti-Pescara, Italy (literal)
Titolo
  • Rat bone regeneration induced by the engraftment of human amniotic fluid stem cells (AFSC) unselected for c-Kit: a pilot study (literal)
Abstract
  • Amniotic fluid contains pluripotent progenitor cells that can differentiate in vitro into cell types from all three germ layers and that do not induce teratomas in vivo(1). Although various stem cell sources have been investigated for bone repair, few comparative reports exist, and cellular distribution and post-implantation viability remain key issues(2). The present study was done with human AFSC expanded in vitro up to the 4th passage in proliferation medium and pre-labelled with a fluorescent cell permeable dye (PKH26) in order to track their in vivo distribution. For in vivo experiments male rats (48-50 week-old; 410-480 g) were injured at the femoral diaphysis in order to produce a 5-7 mm diameter full thickness bone gap. The femoral defect was left untreated (control rats) or filled with HA (natural nanocrystalline carbonated hydroxyapatite-Orthoss®) scaffold alone or loaded with PKH26-labelled AFSC. Three weeks after implantation all animals were sacrificed and femoral specimens were explanted, fixed with 10% buffered formalin, decalcified and processed for conventional paraffin embedding and staining for light microscopy. Both gross anatomy and histological observations revealed a major bone regenerative response in rat specimens treated with HA scaffold alone or supplemented with AFSC. In particular, samples injected with HA plus AFSC displayed the formation of periosteal woven bone and an increased presence of blood vessels in the bone marrow, with still fluorescent AFSC in close proximity. Taken together, these preliminary observations provide evidence that amniotic fluid represents a new and very promising source of engraftable stem cells for treating human orthopaedic disorders (such as non-unions and bone defects) as an alternative therapeutic strategy to bone grafting operations. (literal)
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