Resolution and identification of the protein components of the photosystem II antenna system of higher plants by reversed-phase liquid chromatography with electrospray spectrometric detection (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Resolution and identification of the protein components of the photosystem II antenna system of higher plants by reversed-phase liquid chromatography with electrospray spectrometric detection (Articolo in rivista) (literal)

Anno

• 2000-01-01T00:00:00+01:00 (literal)

Alternative label

• Danilo Corradini, Anna Maria Timperio, Maria Bianchetti, Lello Zolla, Christian Huber (2000)
  Resolution and identification of the protein components of the photosystem II antenna system of higher plants by reversed-phase liquid chromatography with electrospray spectrometric detection
  in Journal of chromatography (Print); Elsevier, Amsterdam (Paesi Bassi)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Danilo Corradini, Anna Maria Timperio, Maria Bianchetti, Lello Zolla, Christian Huber (literal)

Pagina inizio

• 111 (literal)

Pagina fine

• 121 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 886 (literal)

Rivista

• Journal of chromatography (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 11 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Istituto di Cromatografia del CNR, Montelibretti, Roma; Institut für Analytische Chemie und Radiochemie, Leopold-Franzens-Universität, Innsbruck, Austria, Dipartimento di Scienze Ambientali, Università della Tuscia, Viterbo (literal)

Titolo

• Resolution and identification of the protein components of the photosystem II antenna system of higher plants by reversed-phase liquid chromatography with electrospray spectrometric detection (literal)

Abstract

• Reversed-phase liquid chromatography (RPLC) was interfaced to mass spectrometry (MS) with an electrospray ion (ESI) source for the separation and accurate molecular mass determination of the individual intrinsic membrane proteins that comprise the photosystem II (PS II) major light-harvesting complex (LHC II) and minor (CP24, CP26 and CP29) antenna system, whose molecular masses range between 22 000 and 29 000. PS II is a supramolecular complex intrinsic of the thylacoid membrane, which plays the important role in photosynthesis of capturing solar energy, and transferring it to photochemical reaction centers where energy conversion occurs. The protein components of the PS II major and minor antenna systems were extracted from spinach thylacoid membranes and separated using a butyl-silica column eluted by an acetonitrile gradient in 0.05% (v/v) aqueous trifluoroacetic acid. On-line electrospray MS allowed accurate molecular mass determination and identification of the protein components of PS II major and minor antenna system. The proposed RPLC-ESI-MS method holds several advantages over sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the conventional technique for studying membrane proteins, including a better protein separation, mass accuracy, speed and efficiency. (literal)

Editore

• Elsevier (Editore)

Prodotto di

• Istituto di metodologie chimiche (IMC) (Istituto)

Autore CNR

• DANILO CORRADINI (Unità di personale interno)

Insieme di parole chiave

• Keywords of "Resolution and identification of the protein components of the photosystem II antenna system of higher plants by reversed-phase liquid chromatography with electrospray spectrometric detection" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• DANILO CORRADINI (Unità di personale interno)

Prodotto

• Istituto di metodologie chimiche (IMC) (Istituto)
• http://www.cnr.it/ontology/cnr/individuo/modulo/ID6875

Editore di

• Elsevier (Editore)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Journal of chromatography (Rivista)

Insieme di parole chiave di

• Keywords of "Resolution and identification of the protein components of the photosystem II antenna system of higher plants by reversed-phase liquid chromatography with electrospray spectrometric detection" (Insieme di parole chiave)