Separation of tryptophan-derivative enantiomers with iron-free human serum transferrin by capillary zone electrophoresis (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Separation of tryptophan-derivative enantiomers with iron-free human serum transferrin by capillary zone electrophoresis (Articolo in rivista) (literal)

Anno

• 1995-01-01T00:00:00+01:00 (literal)

Alternative label

• KILAR F, FANALI S (1995)
  Separation of tryptophan-derivative enantiomers with iron-free human serum transferrin by capillary zone electrophoresis
  in Electrophoresis (Weinh., Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• KILAR F, FANALI S (literal)

Pagina inizio

• 1510 (literal)

Pagina fine

• 1518 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 16 (literal)

Rivista

• Electrophoresis (Rivista)

Note

• PubMe (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• University of Pecs; IMC-CNR (literal)

Titolo

• Separation of tryptophan-derivative enantiomers with iron-free human serum transferrin by capillary zone electrophoresis (literal)

Abstract

• Enantiomers can be separated by using human serum transferrin as a chiral phase. With the help of the native protein we were able to separate enantiomers with high efficiency, using a low ionic strength 2-(N-morpholino)ethanesulfonic acid (MES) buffer, pH 6, in capillary zone electrophoresis. Tryptophan methyl, ethyl and butyl ester enantiomers - moving towards the cathode at pH 6 - were resolved by passing through an iron-free transferrin zone in coated capillaries. Since the isoelectric point of the iron-free transferrin is a little higher than 6, the protein zone is either not moving in the experiment or is slowly moving towards the anode. Under the simplest experimental conditions the highest resolution was obtained for the butyl ester enantiomers and the lowest for the methyl ester ones. By changing the experimental conditions, however, this order could be reversed. The results indicate that the lengths of the alkyl chains in the enantiomers have a significant effect on the resolution, i.e., on the interaction between the protein and the separands. (literal)

Prodotto di

• Sviluppo di metodologie chimiche avanzate per lo studio di fenomeni delle scienze omiche (PM.P06.019.001) (Modulo)
• Istituto di metodologie chimiche (IMC) (Istituto)

Autore CNR

• SALVATORE FANALI (Persona)

Incoming links:

Prodotto

• Istituto di metodologie chimiche (IMC) (Istituto)
• Sviluppo di metodologie chimiche avanzate per lo studio di fenomeni delle scienze omiche (PM.P06.019.001) (Modulo)

Autore CNR di

• SALVATORE FANALI (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Electrophoresis (Rivista)