Cyclodextrin modified micellar electrokinetic chromatography for the chiral direct resolution of (+), (-)-trans-1,2-dihydrodiol metabolite of chrysene in vitro activated by rat liver microsome S9 fraction (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Cyclodextrin modified micellar electrokinetic chromatography for the chiral direct resolution of (+), (-)-trans-1,2-dihydrodiol metabolite of chrysene in vitro activated by rat liver microsome S9 fraction (Articolo in rivista) (literal)

Anno

• 1995-01-01T00:00:00+01:00 (literal)

Alternative label

• DESIDERIO C, FANALI S, SINIBALDI M, POLCARO C (1995)
  Cyclodextrin modified micellar electrokinetic chromatography for the chiral direct resolution of (+), (-)-trans-1,2-dihydrodiol metabolite of chrysene in vitro activated by rat liver microsome S9 fraction
  in Electrophoresis (Weinh., Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• DESIDERIO C, FANALI S, SINIBALDI M, POLCARO C (literal)

Pagina inizio

• 784 (literal)

Pagina fine

• 788 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 16 (literal)

Rivista

• Electrophoresis (Rivista)

Note

• PubMe (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• IMC-CNR (literal)

Titolo

• Cyclodextrin modified micellar electrokinetic chromatography for the chiral direct resolution of (+), (-)-trans-1,2-dihydrodiol metabolite of chrysene in vitro activated by rat liver microsome S9 fraction (literal)

Abstract

• A ?-cyclodextrin (?-CD) modified electrokinetic micellar capillary chromatography (MEKC) method was used for the enantiomer separation of a racemic trans-1,2-dihydro-1,2-dihydroxy-chrysene (chry-trans-1,2-diOH) mixture. The chiral resolution was strongly influenced by several important parameters: surfactant concentration and addition of organic modifier to the background electrolyte (BGE). An optimized electrophoretic system was used, consisting of the following conditions: 25 mM phosphate buffer, pH 7.8, 50 mM sodium dodecyl sulfate, 20 mM ?-CD, 7.4% v/v 2-propanol as BGE; the applied voltage, 18 kV, corresponded to 37 ?A at a constant temperature of 25°C. This electrophoretic method was applied for monitoring the chry-trans-1,2- diOH enantiomer formation in a real sample, obtained from in vitro metabolic activation of chrysene by phenobarbital-?-naphthoflavone-induced rat microsomes. The (+) and (-) enantiomers were identified by the racemate and the single enantiomer standard addition method and by spectra comparison with the synthetic compound. Under the experimental conditions used for chrysene activation, the (+) optical isomer was the prevailing form. The CD-MEKC system showed high reproducibility and selectivity, allowing a fast and interference-free analysis even of the in vitro metabolic sample extract, without any pretreatment. (literal)

Prodotto di

• Sviluppo di metodologie chimiche avanzate per lo studio di fenomeni delle scienze omiche (PM.P06.019.001) (Modulo)
• Istituto di metodologie chimiche (IMC) (Istituto)

Autore CNR

• CLAUDIA DESIDERIO (Unità di personale interno)
• SALVATORE FANALI (Unità di personale interno)
• MASSIMO SINIBALDI (Persona)
• CHIARA MARCELLA POLCARO (Unità di personale interno)

Incoming links:

Autore CNR di

• CHIARA MARCELLA POLCARO (Unità di personale interno)
• SALVATORE FANALI (Unità di personale interno)
• CLAUDIA DESIDERIO (Unità di personale interno)
• MASSIMO SINIBALDI (Persona)

Prodotto

• Istituto di metodologie chimiche (IMC) (Istituto)
• Sviluppo di metodologie chimiche avanzate per lo studio di fenomeni delle scienze omiche (PM.P06.019.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Electrophoresis (Rivista)