High-Performance Capillary Electrophoretic Determination of Glutathione in Human Lymphocytes (Articolo in rivista)

Type
Label
  • High-Performance Capillary Electrophoretic Determination of Glutathione in Human Lymphocytes (Articolo in rivista) (literal)
Anno
  • 1998-01-01T00:00:00+01:00 (literal)
Alternative label
  • RAGGI MA, MANDRIOLI R, SABBIONI C, MONGIELLO F, MARINI N, FANALI S (1998)
    High-Performance Capillary Electrophoretic Determination of Glutathione in Human Lymphocytes
    in The journal of microcolumn separations (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • RAGGI MA, MANDRIOLI R, SABBIONI C, MONGIELLO F, MARINI N, FANALI S (literal)
Pagina inizio
  • 503 (literal)
Pagina fine
  • 509 (literal)
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  • 10 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Università di Bologna; Università di Bologna; Università di Bologna; Università di Bologna; Università di Bologna; IMC-CNR (literal)
Titolo
  • High-Performance Capillary Electrophoretic Determination of Glutathione in Human Lymphocytes (literal)
Abstract
  • A high-performance capillary electrophoretic method is described which allows for a sensitive and accurate determination of reduced glutathione (GSH) in human lymphocytes. The method makes use of a fused silica capillary with polyacrylamide coating (total length 27 cm, effective length 20 cm, 75 or 50 ?m i.d.). The detection wavelength is 200 nm. The procedure is very simple: the lymphocyte lysate, after suitable filtration, is diluted 1:1 with pH 7.0 phosphate buffer and Ellman 's reagent is subsequently added. This reagent forms a stable adduct with GSH which blocks glutathione into its reduced form and enhances the method sensitivity [limit of detection (LOD) = 5 × 10-7 M]. Reagent excess does not interfere and thus does not need to be eliminated. The method has the advantage of allowing for a rapid and simultaneous determination of both GSH and its oxidized form, glutathione disulfide (GSSG), in a complex matrix such as that of human lymphocytes as well as of requiring only small volumes of the sample. © 1998 John Wiley & Sons, Inc. (literal)
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