Simultaneous stereoselective analysis by capillary electrophoresis of tramadol enantiomers and their main phase I metabolites in urine (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Simultaneous stereoselective analysis by capillary electrophoresis of tramadol enantiomers and their main phase I metabolites in urine (Articolo in rivista) (literal)

Anno

• 1999-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/S0021-9673(99)00028-X (literal)

Alternative label

• RUDAZ S, VEUTHEY JL, DESIDERIO C, FANALI S (1999)
  Simultaneous stereoselective analysis by capillary electrophoresis of tramadol enantiomers and their main phase I metabolites in urine
  in Journal of chromatography (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• RUDAZ S, VEUTHEY JL, DESIDERIO C, FANALI S (literal)

Pagina inizio

• 227 (literal)

Pagina fine

• 237 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 846 (literal)

Rivista

• Journal of chromatography (Rivista)

Note

• PubMe (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• University of Geneve; University of Geneve; IMC-CNR; IMC-CNR (literal)

Titolo

• Simultaneous stereoselective analysis by capillary electrophoresis of tramadol enantiomers and their main phase I metabolites in urine (literal)

Abstract

• Capillary zone electrophoresis was successfully applied to the enantiomeric resolution of racemic tramadol and its six phase I metabolites using carboxymethylated ?-cyclodextrin (CMB) added to the background electrolyte (BGE). Baseline resolution of tramadol and its metabolites was obtained in less than 30 min using a 50 mM phosphate buffer (pH 2.5) containing 5 mM of CMB. Chiral determinations of tramadol and its main three metabolites, O-demethyltramadol (M1), N-demethyltramadol (M2) and O-demethyl-N-demethyltramadol (M5), were performed in urine after a simple double liquid-liquid extraction of 200 ?l of biological material. In the tested concentration range (0.5-20 ?g/ml, except for M2: 0.5-10 ?g/ml) coefficients of correlation superior than 0.994 were obtained. Within-day variation determined on three different concentrations for each enantiomers showed accuracies ranging from 95.4% to 103.2%. The relative standard deviation (RSD) of these assays was determined to be less than 10.0%. Day-to-day variation presented accuracies ranging from 96.3% to 106.5% with a RSD less than 9.0%. After oral administration of 100 mg of tramadol hydrochloride to an healthy volunteer, the urinary excretion was monitored during 30 h. About 15% of the dose was excreted as unchanged tramadol. The enantiomeric ratios of all the excreted analytes, T, M1, M2 and M5, were found to be very different to 1.0, showing that a stereoselective metabolism of tramadol clearly occurred. (literal)

Prodotto di

• Sviluppo di metodologie chimiche avanzate per lo studio di fenomeni delle scienze omiche (PM.P06.019.001) (Modulo)
• Istituto di metodologie chimiche (IMC) (Istituto)

Autore CNR

• SALVATORE FANALI (Unità di personale interno)
• CLAUDIA DESIDERIO (Unità di personale interno)

Incoming links:

Prodotto

• Istituto di metodologie chimiche (IMC) (Istituto)
• Sviluppo di metodologie chimiche avanzate per lo studio di fenomeni delle scienze omiche (PM.P06.019.001) (Modulo)

Autore CNR di

• SALVATORE FANALI (Unità di personale interno)
• CLAUDIA DESIDERIO (Unità di personale interno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Journal of chromatography (Rivista)