Proliferation and activation of bronchial epithelial cells in corticosteroid-dependent asthma (Articolo in rivista)

Type
Label
  • Proliferation and activation of bronchial epithelial cells in corticosteroid-dependent asthma (Articolo in rivista) (literal)
Anno
  • 2001-01-01T00:00:00+01:00 (literal)
Alternative label
  • 1.Vignola AM, 1.Chiappara G, 1.Siena L, 1.Bruno A, 1.Gagliardo R, 2.Merendino AM, 3.Polla BS, 4.Arrigo AP, 1.Bonsignore G, 5.Bousquet J, 5.Chanez P (2001)
    Proliferation and activation of bronchial epithelial cells in corticosteroid-dependent asthma
    in The journal of allergy and clinical immunology (Online)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • 1.Vignola AM, 1.Chiappara G, 1.Siena L, 1.Bruno A, 1.Gagliardo R, 2.Merendino AM, 3.Polla BS, 4.Arrigo AP, 1.Bonsignore G, 5.Bousquet J, 5.Chanez P (literal)
Pagina inizio
  • 738 (literal)
Pagina fine
  • 746 (literal)
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  • 108 (literal)
Rivista
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  • 5 (literal)
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  • 1. I.B.I.M., CNR, Palermo, Italy 2. Clinica Pneumologica, Università degli Studi di Palermo 3. Laboratoire de Physiologie Respiratoire, UFR Cochin Port-Royal, Université Paris V 4.Laboratoire du Stress Cellulaire, CGMC, CNRS UMR 5534, Université Claude Bernard, Lyon 5.Clinique des Maladies Respiratoires and INSERM U 454, Hôpital Arnaud de Villeneuve, Montpellier (literal)
Titolo
  • Proliferation and activation of bronchial epithelial cells in corticosteroid-dependent asthma (literal)
Abstract
  • Background: Structural and functional characteristics of bronchial epithelial cells in corticosteroid-dependent asthma are unknown. Objective: In bronchial biopsy specimens from 16 control, 9 untreated asthmatic, 9 inhaled corticosteroid-treated asthmatic, and 19 corticosteroid-dependent asthmatic subjects, we evaluated epithelium morphology and patterns of cell apoptosis, proliferation, and activation. Methods:We used the terminal deoxynucleotidyl-mediated dUTP nick end labeling (TUNEL) technique to study apoptosis. Immunohistochemistry was used to evaluate the expression of molecules related to apoptosis (such as Bcl-2 and P53), cell proliferation (PCNA), and cell activation (NF?B and CD40/CD40-L). Results: Epithelium thickness was higher in corticosteroiddependent asthmatic and control subjects than in inhaled corticosteroid- treated and untreated asthmatic subjects (P < .0001 and P < .0003). Very few TUNEL-positive epithelial cells were found in the 4 groups. Bcl-2 expression was higher in all groups of asthmatic subjects than in controls (P < .001). In corticosteroid-dependent asthmatic subjects, PCNA, NF?B, and CD40-L expression was higher than in inhaled corticosteroid- treated asthmatic (P < .001), untreated asthmatic (P < .001 and P < .04), and control (P < .01) subjects. CD40 expression was greater in corticosteroid-dependent asthmatic and untreated asthmatic subjects than in inhaled corticosteroid- treated asthmatic subjects (P < .0001 and P < .0006) and controls (P < .02 and P < .03). In corticosteroid-dependent asthma, PCNA expression was correlated with the epithelium thickness (P < .007). Conclusion: This study shows that in bronchial epithelial cells of corticosteroid-dependent asthma, markers of cell survival and proliferation are coexpressed with markers of cell activation, suggesting that in this disease epithelium repair is associated with a persistent activation state of epithelial cells. (J Allergy Clin Immunol 2001;108:738-46.) (literal)
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