15-Lipoxygenase expression and 15(S)-hydroxyeicoisatetraenoic acid release and reincorporation in induced sputum of asthmatic subjects (Articolo in rivista)

Type
Label
  • 15-Lipoxygenase expression and 15(S)-hydroxyeicoisatetraenoic acid release and reincorporation in induced sputum of asthmatic subjects (Articolo in rivista) (literal)
Anno
  • 2000-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1067/mai.2000.105122 (literal)
Alternative label
  • Mirella Profita, PhD,a Angelo Sala, PhD,c Loredana Riccobono, PhD,a Alessandra Paternò, PhD,a Angela Mirabella, PhD,b Anna Bonanno, PhD,a Daniela Guerrera, MD,a Elisabetta Pace, MD,a Giovanni Bonsignore, MD,a,b Jean Bousquet, MD, PhD,d and Antonio M.Vignola, MD, PhDa (2000)
    15-Lipoxygenase expression and 15(S)-hydroxyeicoisatetraenoic acid release and reincorporation in induced sputum of asthmatic subjects
    in Journal of allergy and clinical immunology
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Mirella Profita, PhD,a Angelo Sala, PhD,c Loredana Riccobono, PhD,a Alessandra Paternò, PhD,a Angela Mirabella, PhD,b Anna Bonanno, PhD,a Daniela Guerrera, MD,a Elisabetta Pace, MD,a Giovanni Bonsignore, MD,a,b Jean Bousquet, MD, PhD,d and Antonio M.Vignola, MD, PhDa (literal)
Pagina inizio
  • 711 (literal)
Pagina fine
  • 716 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 105 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • a Istituto di Fisiopatologia Respiratoria, Consiglio delle Ricerche, and the b Istituto di Medicina Generale e Pneumologia, University of Palermo, Palermo, Italy, the c Center for Cardiopulmonary Pharmacology, University of Milan, Milan, Italy, and the d Institut National de la Santé et de la Recherche Médicale U-454, Montpellier, France. (literal)
Titolo
  • 15-Lipoxygenase expression and 15(S)-hydroxyeicoisatetraenoic acid release and reincorporation in induced sputum of asthmatic subjects (literal)
Abstract
  • BACKGROUND: Recent evidence shows that 15(S)-hydroxy-eicoisatetraenoic acid (15[S]-HETE) can be released and rapidly reincorporated into cellular lipids. These mechanisms exert several immunoregulatory functions that may be relevant in airway inflammation. OBJECTIVE: Our purpose was to evaluate the levels of both soluble and cell-associated 15(S)-HETE and to examine 15-lipoxygenase (15-LO) messenger RNA (mRNA) expression in sputum samples obtained from 10 control and 18 asthmatic subjects. METHODS: Levels of 15(S)-HETE were measured by reverse-phase HPLC separation followed by RIA in supernatants and in cell membrane-extracted phospholipids after acid hydrolysis. 15-LO mRNA was evaluated by primed in situ hybridization (PRINS). Combined immunocytochemistry and PRINS was used to identify the phenotype of cells bearing 15-LO transcripts. RESULTS: Levels of both soluble and cell-associated 15(S)-HETE were higher in asthmatic than in control subjects (P <.0001). The percentage of cells expressing 15-LO mRNA was higher in asthmatic than in control subjects (P <.01). On double staining for specific cell-type markers and 15-LO mRNA, macrophages were the major source for 15-LO. CONCLUSION: This study shows that the induced sputum technique allows the evaluation of 15-LO activity and that soluble, cell-associated 15(S)-HETE and 15-LO levels are higher in asthmatic than in control subjects. In addition, this study indicates that, in induced sputum, airway macrophages are the major source of 15(S)-HETE in asthma. (literal)
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