Identification of early induced genes upon water deficit in potato cell cultures by cDNA-AFLP (Articolo in rivista)

Type
Label
  • Identification of early induced genes upon water deficit in potato cell cultures by cDNA-AFLP (Articolo in rivista) (literal)
Anno
  • 2013-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1007/s10265-012-0505-7 (literal)
Alternative label
  • Alfredo Ambrosone, Michele Di Giacomo, Antonella Leone, M. Stefania Grillo, Antonello Costa (2013)
    Identification of early induced genes upon water deficit in potato cell cultures by cDNA-AFLP
    in Journal of plant research
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Alfredo Ambrosone, Michele Di Giacomo, Antonella Leone, M. Stefania Grillo, Antonello Costa (literal)
Pagina inizio
  • 169 (literal)
Pagina fine
  • 178 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 126 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
  • 10 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Istituto di genetica vegetale (literal)
Titolo
  • Identification of early induced genes upon water deficit in potato cell cultures by cDNA-AFLP (literal)
Abstract
  • Abstract For plant cells in the early phases of water stress exposure, the genes induced under such conditions play a key role in detecting and responding to water deficit. In this study, potato cell suspensions were used as a sim- plified model system to dissect early molecular changes upon low water potential. In particular, the cDNA-ampli- fied fragment length polymorphism approach was used to capture genes rapidly activated in potato cell cultures in response to water deficit induced by short-term exposure (up to 1 h) to polyethylene glycol. Selective amplifications with 38 primer combinations allowed the visualization of about 167 transcript-derived fragments (TDFs) differen- tially expressed upon exposure to low water potential. The gene expression pattern of 18 up-regulated genes was further investigated by semi-quantitative reverse trans- criptase polymerase chain reaction analysis. Sequencing and similarity analysis revealed that TDFs present homol- ogies chiefly with proteins involved in chaperone activity and protein degradation (hsps, proteinase precursor), in protein synthesis (elongation factor, ribosomal proteins) and in the ROS scavenging pathway (phenylalanine ammonia-lyase, peroxidase). Our findings might contribute to describe the potential role of genes activated in the early phases of plant response to drought. (literal)
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