A new miniaturized multiarrays biosensor system for fluorescence detection (Articolo in rivista)

Type
Label
  • A new miniaturized multiarrays biosensor system for fluorescence detection (Articolo in rivista) (literal)
Anno
  • 2007-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1088/0953-8984/19/39/395006 (literal)
Alternative label
  • Tibuzzi Arianna (1,4), Rea Giuseppina (2), Pezzotti Gianni (1), Esposito Dania (2), Johanningmeier Udo (3) and Giardi Maria Teresa (2) (2007)
    A new miniaturized multiarrays biosensor system for fluorescence detection
    in Journal of physics. Condensed matter (Online)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Tibuzzi Arianna (1,4), Rea Giuseppina (2), Pezzotti Gianni (1), Esposito Dania (2), Johanningmeier Udo (3) and Giardi Maria Teresa (2) (literal)
Pagina inizio
  • 395006 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni
  • Conference: International School and Workshop on Nanoscience and Nanotechnologh Location: Rome, ITALY Date: NOV 06-09, 2006 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url
  • http://iopscience.iop.org (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 19 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
  • 12 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 39 (literal)
Note
  • Scopu (literal)
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • 1) Biosensor S.r.l., Viale Tivoli Km 18,642, 00018, Palombara Sabina, Rome, Italy; 2) National Research Council--IC, Department of Food and Agriculture, Research Area of Rome 1, Via Salaria Km 29,300, 00016 Monterotondo Scalo, Rome, Italy; 3) Martin-Luther-University of Halle, Institute for Plant Physiology, Weinbergweg 10, D-06120 Halle, Germany; (literal)
Titolo
  • A new miniaturized multiarrays biosensor system for fluorescence detection (literal)
Abstract
  • A miniaturized biosensor-based optical instrument has been designed and fabricated for multiarray fluorescence measurements of several biomediators in series, with applications in environmental monitoring and agrofood analysis. It is a multicell system featuring two arrays of five static cells (1 × 1 × 2 cm3) which are sealed to avoid contamination. Every cell is made up by two modular sections: the bottom compartment with optical LED light excitations and a photodiode detector for fluorescence emission capture, and the top biocompatible compartment where the biosample is deposited. The system (0.250 kg without batteries and case, 100 × 100 × 150 mm3 internal case dimensions) is equipped with electronic control boards, a flash memory card for automatic data storage, and internal batteries, thus being portable and versatile. The instrument allows one to perform simultaneous and multiparametric analyses and offers a large applicability in biosensor technology. The first prototype has been implemented with genetically modified oxygenic photosynthetic algae that were employed in the instrument experimental testing by monitoring pesticide pollution in water. Pesticides modify the photosystem II (PSII) activity in terms of fluorescence quenching. The PSII complex features a natural nanostructure and can be considered a sophisticated molecular device. Results from measurements employing several PSII mutants and six different pesticides at increasing concentrations and incubation times are presented and discussed. (literal)
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