Enantioseparation of S-carboxymethylcysteine and N-acetamidocarboxymethylcysteine by capillary electrophoresis using vancomycin (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Enantioseparation of S-carboxymethylcysteine and N-acetamidocarboxymethylcysteine by capillary electrophoresis using vancomycin (Articolo in rivista) (literal)

Anno

• 2001-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1002/1615-9314(20010901)24:9<789::AID-JSSC789>3.0.CO;2-J (literal)

Alternative label

• FANALI S, CARTONI C, ATURKI Z (2001)
  Enantioseparation of S-carboxymethylcysteine and N-acetamidocarboxymethylcysteine by capillary electrophoresis using vancomycin
  in Journal of separation science (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• FANALI S, CARTONI C, ATURKI Z (literal)

Pagina inizio

• 794 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 24 (literal)

Rivista

• Journal of separation science (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 789 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• IMC-CNR (literal)

Titolo

• Enantioseparation of S-carboxymethylcysteine and N-acetamidocarboxymethylcysteine by capillary electrophoresis using vancomycin (literal)

Abstract

• Racemic S-carboxymethylcysteine (DF-1794Y) and N-acetamido S-carboxymethylcystein (DF-1796A) were resolved into their enantiomers by capillary electrophoresis using vancomycin as a chiral selector. Electrophoretic runs were carried out in a polyacrylamide-coated capillary filled with a background electrolyte composed of sorbic acid/histidine (pH=4.5-6.5) and the appropriate concentration of vancomycin. The presence of the chiral selector (CS) in the detector path was avoided by using the partial filling-countercurrent method, where the CS filled only part of the capillary and was moving in the opposite direction to the analytes. Due to the relatively low absorption of the enantiomers studied, indirect UV detection was used in order to increase the sensitivity of the method. For method optimization the effect of several experimental parameters such as vancomycin concentration, buffer pH, and organic modifer type and concentration on enantioresolution and migration time were studied. The migration order was verified only for S-carboxymethylcysteine by injecting a mixture containing the racemic DF-1794Y spiked with the enantiomer S-carboxymethyl-L-cysteine. The L-isomer was found to migrate first. (literal)

Prodotto di

• Sviluppo di metodologie chimiche avanzate per lo studio di fenomeni delle scienze omiche (PM.P06.019.001) (Modulo)
• Istituto di metodologie chimiche (IMC) (Istituto)

Autore CNR

• ZEINEB ATURKI (Unità di personale interno)
• SALVATORE FANALI (Unità di personale interno)

Incoming links:

Autore CNR di

• ZEINEB ATURKI (Unità di personale interno)
• SALVATORE FANALI (Unità di personale interno)

Prodotto

• Istituto di metodologie chimiche (IMC) (Istituto)
• Sviluppo di metodologie chimiche avanzate per lo studio di fenomeni delle scienze omiche (PM.P06.019.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Journal of separation science (Rivista)