The Interaction of Kanamycin A with the Bacterial Ribosomal A-Site: Effect of Cu(II) on the Adduct (Articolo in rivista)

Type
Label
  • The Interaction of Kanamycin A with the Bacterial Ribosomal A-Site: Effect of Cu(II) on the Adduct (Articolo in rivista) (literal)
Anno
  • 2008-01-01T00:00:00+01:00 (literal)
Alternative label
  • Balenci D, Bernardi F, Cellai L, D'Amelio N, Gaggelli E, Gaggelli N, Molteni E, Valensin G. (2008)
    The Interaction of Kanamycin A with the Bacterial Ribosomal A-Site: Effect of Cu(II) on the Adduct
    in ChemBioChem (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Balenci D, Bernardi F, Cellai L, D'Amelio N, Gaggelli E, Gaggelli N, Molteni E, Valensin G. (literal)
Pagina inizio
  • 114 (literal)
Pagina fine
  • 123 (literal)
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  • 9 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Balenci D. -Department of Chemistry, University of Siena Bernardi F. -Department of Chemistry, University of Siena Cellai L. - IC-CNR, Roma D'Amelio N. -Department of Chemistry, University of Siena Gaggelli E. -Department of Chemistry, University of Siena Gaggelli N. -Department of Chemistry, University of Siena Molteni E. -Department of Chemistry, University of Siena Valensin G. -Department of Chemistry, University of Siena (literal)
Titolo
  • The Interaction of Kanamycin A with the Bacterial Ribosomal A-Site: Effect of Cu(II) on the Adduct (literal)
Abstract
  • The solution structure of kanamycin A interacting with a ribosomal A-site fragment was solved by transferred-NOE techniques and found to agree with the structure of the complex observed in the crystal. Despite the fast exchange conditions found for the interaction, the bound form was identified by NOESY spectroscopy. At 600 MHz, NOE effects are only observed for the RNA-associated antibiotic. Dissociation constants were measured by NMR spectroscopy for two sites of interaction (K(d1)=150+/-40 microM; K(d2)=360+/-50 microM). Furthermore, the effects of the Cu(II) ion on the antibiotic, on the RNA fragment that mimics the bacterial ribosomal A site, and on the complex formed between these two entities were analyzed. The study led to the proposal of a model that localizes the copper ion within the kanamycin-RNA complex. (literal)
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