Distinct mechanisms regulating gene expression co-exist within the fermentative pathways in Chlamydomonas reinhardtii (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Distinct mechanisms regulating gene expression co-exist within the fermentative pathways in Chlamydomonas reinhardtii (Articolo in rivista) (literal)

Anno

• 2012-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1100/2012/565047 (literal)

Alternative label

• Larisa Angela Swirsky Whitney (1), Giacomo Novi (2), Pierdomenico Perata (2), Elena Loreti (3) (2012)
  Distinct mechanisms regulating gene expression co-exist within the fermentative pathways in Chlamydomonas reinhardtii
  in TheScientificWorldjournal
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Larisa Angela Swirsky Whitney (1), Giacomo Novi (2), Pierdomenico Perata (2), Elena Loreti (3) (literal)

Rivista

• TheScientificWorldjournal (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 9 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• (1) Department of Crop Plant Biology, University of Pisa, Via Mariscoglio 34, 56124 Pisa, Italy. (2) PlantLab, Institute of Life Sciences, Scuola Superiore Sant'Anna, Via Mariscoglio 34, 56124 Pisa, Italy. (3) Institute of Agricultural Biology and Biotechnology, National Research Council, Via Moruzzi 1, 56100 Pisa, Italy. (literal)

Titolo

• Distinct mechanisms regulating gene expression co-exist within the fermentative pathways in Chlamydomonas reinhardtii (literal)

Abstract

• Under dark anoxia, the unicellular green algae Chlamydomonas reinhardtii may produce hydrogen by means of its hydrogenase enzymes, in particular HYD1, using reductants derived from the degradation of intercellular carbon stores. Other enzymes belonging to the fermentative pathways compete for the same reductants. A complete understanding of the mechanisms determining the activation of one pathway rather than another will help us engineer Chlamydomonas for fermentative metabolite production, including hydrogen. We examined the expression pattern of the fermentative genes PDC3, LDH1, ADH2, PFL1, and PFR1 in response to day-night cycles, continuous light, continuous darkness, and low or high oxygen availability, which are all conditions that vary on a regular basis in Chlamydomonas' natural environment. We found that all genes except PFL1 show daily fluctuations in expression, and that PFR1 differentiated itself from the others in that it is clearly responsive to low oxygen, where as PDC3, LDH1, and ADH2 are primarily under diurnal regulation. Our results provide evidence that there exist at least three different regulatory mechanisms within the fermentative pathways and suggest that the fermentative pathways are not redundant but rather that availability of a variety of pathways allows for a differential metabolic response to different environmental conditions. (literal)

Prodotto di

• Basi molecolari, fisiologiche e cellulari delle produzioni vegetali. (AG.P01.001.001) (Modulo)
• Institute of agricultural biology and biotechnology (IBBA) (Istituto)

Autore CNR

• ELENA LORETI (Unità di personale interno)

Insieme di parole chiave

• Keywords of "Distinct mechanisms regulating gene expression co-exist within the fermentative pathways in Chlamydomonas reinhardtii" (Insieme di parole chiave)

Incoming links:

Prodotto

• Institute of agricultural biology and biotechnology (IBBA) (Istituto)
• Basi molecolari, fisiologiche e cellulari delle produzioni vegetali. (AG.P01.001.001) (Modulo)

Autore CNR di

• ELENA LORETI (Unità di personale interno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• TheScientificWorldjournal (Rivista)

Insieme di parole chiave di

• Keywords of "Distinct mechanisms regulating gene expression co-exist within the fermentative pathways in Chlamydomonas reinhardtii" (Insieme di parole chiave)