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Role of ionic liquids in protein refolding: native/fibrillar versus treated lysozyme (Articolo in rivista)

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Role of ionic liquids in protein refolding: native/fibrillar versus treated lysozyme (Articolo in rivista) (literal)

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Sara Mangialardo , Lorenzo Gontrani , Francesca Leonelli , Ruggero Caminiti and Paolo Postorino (2012)
Role of ionic liquids in protein refolding: native/fibrillar versus treated lysozyme
in RSC advances; Royal Society of Chemistry, Cambridge (Regno Unito)
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Sara Mangialardo , Lorenzo Gontrani , Francesca Leonelli , Ruggero Caminiti and Paolo Postorino (literal)

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Department of Physics, Universita` di Roma Sapienza, Italy. CNR-ISM Tor Vergata, Roma, Italy Department of Chemistry, Universita` di Roma Sapienza, Italy. Department of Physics, Universita` di Roma Sapienza, Italy. (literal)

Titolo

Role of ionic liquids in protein refolding: native/fibrillar versus treated lysozyme (literal)

Abstract

Several ionic liquids (ILs) are known to revert aggregation processes and to improve the in vitro refolding of denatured/fibrillar proteins. Here, Raman spectroscopy is exploited to verify the refolding capability of several ammonium-based ILs and to identify the microscopic signatures of the structural rearrangements induced by the interaction of ILs with fibrillar lysozyme. We collected and carefully analyzed spectra from native, fibrillar and ILs-treated fibrillar lysozyme to follow the microscopic process induced by ILs. These allowed us to identify different mechanisms of interaction depending on the length of the cation alkyl chain. A clear refolding effect was observed with EAN, as well as a tendency of the longer alkyl chain (PAN and BAN) of dissolving the fibril packing. A specific interaction mainly affecting the aromatic residues was identified for MEOAN (a long chain ILs with an ether group). The whole of the results, thus, provides new and detailed information on the ILs-protein interaction and shows Raman spectroscopy as a simple, reliable and effective diagnostic technique in this field. (literal)

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