http://www.cnr.it/ontology/cnr/individuo/prodotto/ID194095

Human chorionic villus mesenchymal stromal cells reveal strong endothelial conversion properties (Articolo in rivista)

Type

Prodotto della ricerca (Classe)
Articolo in rivista (Classe)

Label

Human chorionic villus mesenchymal stromal cells reveal strong endothelial conversion properties (Articolo in rivista) (literal)

Anno

2012-01-01T00:00:00+01:00 (literal)

Alternative label

Viviana Meraviglia a; Matteo Vecellio b-a; Annalisa Grasselli c, Marco Baccarin d; Antonella Farsetti e-f; Maurizio C. Capogrossi g; Giulio Pompilio b-a; Domenico A. Coviello h; Carlo Gaetano g; Marina Di Segni d; Alessandra Rossini b-a-n (2012)
Human chorionic villus mesenchymal stromal cells reveal strong endothelial conversion properties
in Differentiation (Lond.)
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Viviana Meraviglia a; Matteo Vecellio b-a; Annalisa Grasselli c, Marco Baccarin d; Antonella Farsetti e-f; Maurizio C. Capogrossi g; Giulio Pompilio b-a; Domenico A. Coviello h; Carlo Gaetano g; Marina Di Segni d; Alessandra Rossini b-a-n (literal)

Pagina inizio

260 (literal)

Pagina fine

270 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

83 (literal)

Rivista

Differentiation (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

5 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

a Laboratorio di Biologia Vascolare e Medicina Rigenerativa, CentroCardiologicoMonzino, IRCCS, Milano, Italy. b Dipartimento di Scienze Cardiovascolari, Universit?à degli Studi di Milano, Milano, Italy. c Unit?a Cardiologica, INRCA, IRCCS, Ancona, Italy. d Laboratorio di Genetica Medica - Fondazione IRCCS Ca' Granda - Ospedale Maggiore Policlinico, Milano, Italy. e Istituto Nazionale Tumori Regina Elena, Roma, Italy. f Istituto di Neurobiologia e Medicina Molecolare, Consiglio Nazionale delle Ricerche (CNR), Roma, Italy. g Laboratorio diPatologiaVascolare,IstitutoDermopaticodell'Immacolata,IRCCS,Roma,Italy h Laboratorio diGeneticaUmana,OspedaliGalliera,Genova,Italy (literal)

Titolo

Human chorionic villus mesenchymal stromal cells reveal strong endothelial conversion properties (literal)

Abstract

Chorion, amnion and villi are reservoirs of mesenchymal stromal cells (StC) and the hypothesis that StC from fetal tissues retain higher plasticity compared to adult StC has been suggested. Aimed at investigating this aspect, a series of in vitro experiments were performed with StC isolated from first trimester human chorionic villi (CVStC). CVStC were cultured in: (i) standard mesenchymal medium (MM) and (ii) AmniomaxII® (AM), specifically designed to grow amnion-derived cells in prenatal diagnostic procedures. Cells were then exposed to distinct differentiation treatments and distinguished according to morphology, immunophenotype and molecular markers. Human StC obtained from adult bone marrow (BMStC) were used as control. CVStC cultured either in MM or AM presented stromal morphology and immunophenotype, were negative for pluripotency factors (Nanog, Oct-4 and Sox-2), lacked detectable telomerase activity and retained high genomic stability. In AM, however, CVStC exhibited a faster proliferation rate compared to BMStC or CVStC kept in MM. During differentiation, CVStC were less efficient than BMStC in acquiring adipocytes and osteocytes features; the cardiomyogenic conversion occurred at low efficiency in both cell types. Remarkably, in the presence of pro-angiogenic factors, CVStC reprogrammed toward an endothelial-like phenotype at significantly higher efficiency than BMStC. This effect was particularly evident in CVStC expanded in AM. Mechanistically, the reduced CVStC expression of anti-angiogenic microRNA could support this process. The present study demonstrates that, despite of fetal origin, CVStC exhibit restricted plasticity, distinct from that of BMStC and predominantly directed toward the endothelial lineage. (literal)

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