http://www.cnr.it/ontology/cnr/individuo/prodotto/ID193942
Simultaneous determination of lactate and pyruvate in human sweat using reversed-phase high-performance liquid chromatography: a noninvasive approach (Articolo in rivista)
- Type
- Label
- Simultaneous determination of lactate and pyruvate in human sweat using reversed-phase high-performance liquid chromatography: a noninvasive approach (Articolo in rivista) (literal)
- Anno
- 2012-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1002/bmc.2713 (literal)
- Alternative label
Biagi, Simona; Ghimenti, Silvia; Onor, Massimo; Bramanti, Emilia (2012)
Simultaneous determination of lactate and pyruvate in human sweat using reversed-phase high-performance liquid chromatography: a noninvasive approach
in BMC. Biomedical chromatography
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Biagi, Simona; Ghimenti, Silvia; Onor, Massimo; Bramanti, Emilia (literal)
- Pagina inizio
- Pagina fine
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
- Rivista
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
- Note
- ISI Web of Science (WOS) (literal)
- Scopus (literal)
- PubMed (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- 1. UOS Pisa, Natl Res Council Italy, CNR, IPCF, I-56124 Pisa, Italy
2. Dipartimento Chim & Chim Ind, I-56127 Pisa, Italy
3. UOS Pisa, Natl Res Council Italy, CNR, ICCOM, I-56124 Pisa, Italy (literal)
- Titolo
- Simultaneous determination of lactate and pyruvate in human sweat using reversed-phase high-performance liquid chromatography: a noninvasive approach (literal)
- Abstract
- The simultaneous determination of lactate and pyruvate in sweat has been performed using reversed phase high-performance liquid chromatography (RP-HPLC) with UV detection at 220 nm. The calibration curves were linear in the investigated range 0.3 - 350 mm of lactate, 0.003- 1 mm of pyruvate. The sensitivity was good with a limit of detection of 0.03 mm for lactate and 0.001 mm for pyruvate. Recoveries evaluated for the entire procedure were 102 ± 0.1 and 96 ± 0.1for lactate and pyruvate, respectively. The method was successfully applied to analysis of sweat in 8 athletes at rest (pilocarpine sweating) and during physical exercise. (literal)
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