Simultaneous determination of lactate and pyruvate in human sweat using reversed-phase high-performance liquid chromatography: a noninvasive approach (Articolo in rivista)

Type
Label
  • Simultaneous determination of lactate and pyruvate in human sweat using reversed-phase high-performance liquid chromatography: a noninvasive approach (Articolo in rivista) (literal)
Anno
  • 2012-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1002/bmc.2713 (literal)
Alternative label
  • Biagi, Simona; Ghimenti, Silvia; Onor, Massimo; Bramanti, Emilia (2012)
    Simultaneous determination of lactate and pyruvate in human sweat using reversed-phase high-performance liquid chromatography: a noninvasive approach
    in BMC. Biomedical chromatography
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Biagi, Simona; Ghimenti, Silvia; Onor, Massimo; Bramanti, Emilia (literal)
Pagina inizio
  • 1408 (literal)
Pagina fine
  • 1415 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 26 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 11 (literal)
Note
  • ISI Web of Science (WOS) (literal)
  • Scopus (literal)
  • PubMed (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • 1. UOS Pisa, Natl Res Council Italy, CNR, IPCF, I-56124 Pisa, Italy 2. Dipartimento Chim & Chim Ind, I-56127 Pisa, Italy 3. UOS Pisa, Natl Res Council Italy, CNR, ICCOM, I-56124 Pisa, Italy (literal)
Titolo
  • Simultaneous determination of lactate and pyruvate in human sweat using reversed-phase high-performance liquid chromatography: a noninvasive approach (literal)
Abstract
  • The simultaneous determination of lactate and pyruvate in sweat has been performed using reversed phase high-performance liquid chromatography (RP-HPLC) with UV detection at 220 nm. The calibration curves were linear in the investigated range 0.3 - 350 mm of lactate, 0.003- 1 mm of pyruvate. The sensitivity was good with a limit of detection of 0.03 mm for lactate and 0.001 mm for pyruvate. Recoveries evaluated for the entire procedure were 102 ± 0.1 and 96 ± 0.1for lactate and pyruvate, respectively. The method was successfully applied to analysis of sweat in 8 athletes at rest (pilocarpine sweating) and during physical exercise. (literal)
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