Multiplex dipstick immunoassay for semi-quantitative determination of Fusarium mycotoxins in cereals (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Multiplex dipstick immunoassay for semi-quantitative determination of Fusarium mycotoxins in cereals (Articolo in rivista) (literal)

Anno

• 2012-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/j.aca.2011.12.060 (literal)

Alternative label

• Lattanzio V.M.T., Nivarlet N., Lippolis V., Della Gatta S., Huet A.C., Delahaut P., Granier B., Visconti A (2012)
  Multiplex dipstick immunoassay for semi-quantitative determination of Fusarium mycotoxins in cereals
  in Analytica chimica acta (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Lattanzio V.M.T., Nivarlet N., Lippolis V., Della Gatta S., Huet A.C., Delahaut P., Granier B., Visconti A (literal)

Pagina inizio

• 99 (literal)

Pagina fine

• 108 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 718 (literal)

Rivista

• Analytica chimica acta (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Ispa-cnr (literal)

Titolo

• Multiplex dipstick immunoassay for semi-quantitative determination of Fusarium mycotoxins in cereals (literal)

Abstract

• A multiplex dipstick immunoassay based method for the simultaneous determination of major Fusarium toxins, namely zearalenone, T-2 and HT-2 toxins, deoxynivalenol and fumonisins in wheat, oats and maize has been developed. The dipstick format was based on an indirect competitive approach. Four test lines (mycotoxin-BSA conjugates) and one control line were located on the strip membrane. Labelled antibodies were freeze-dried within the microwell. Two matrix-related sample preparation protocols have been developed for wheat/oats (not containing fumonisins) and maize (containing fumonisins) respectively. The use of a methanol/water mixture for sample preparation allowed recoveries in the range 73-109% for all mycotoxins in all tested cereals, with relative standard deviation less than 10%. The optimized immunoassay was able to detect target mycotoxins at cut off levels equal to 80% of EU maximum permitted levels, i.e. 280, 400, 1400 and 3200 ?g kg-1, respectively, for zearalenone, T-2/HT-2 toxins, deoxynivalenol and fumonisins in maize, and 80, 400 and 1400 ?g kg-1, respectively, for zearalenone, T-2/HT-2 toxins and deoxynivalenol in wheat and oats. Analysis of naturally contaminated samples resulted in a good agreement between multiplex dipstick and validated confirmatory LC-MS/MS. The percentage of false positive results was less than or equal to 13%, whereas no false negative results were obtained. Data on the presence/absence of 6 mycotoxins at levels close to EU regulatory levels were obtained within 30 min. The proposed immunoassay protocol is rapid, inexpensive, easy-to-use and fit for purpose of rapid screening of mycotoxins in cereals. (literal)

Prodotto di

• Metodi innovativi per l'analisi e la riduzione di micotossine, funghi tossigeni ed allergeni nei prodotti agroalimentari (AG.P05.008.002) (Modulo)
• Istituto di scienze delle produzioni alimentari (ISPA) (Istituto)

Autore CNR

• VINCENZO LIPPOLIS (Persona)
• Stefania Della Gatta (Unità di personale esterno)
• VERONICA MARIA TERESA LATTANZIO (Unità di personale interno)
• ANGELO VISCONTI (Unità di personale interno)

Incoming links:

Autore CNR di

• VINCENZO LIPPOLIS (Persona)
• ANGELO VISCONTI (Unità di personale interno)
• Stefania Della Gatta (Unità di personale esterno)
• VERONICA MARIA TERESA LATTANZIO (Unità di personale interno)

Prodotto

• Istituto di scienze delle produzioni alimentari (ISPA) (Istituto)
• Metodi innovativi per l'analisi e la riduzione di micotossine, funghi tossigeni ed allergeni nei prodotti agroalimentari (AG.P05.008.002) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Analytica chimica acta (Rivista)