Mobilized blood CD34+ cells transduced and selected with a clinically applicable protocol reconstitute lymphopoiesis in SCID-Hu mice. (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Mobilized blood CD34+ cells transduced and selected with a clinically applicable protocol reconstitute lymphopoiesis in SCID-Hu mice. (Articolo in rivista) (literal)

Anno

• 2004-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1089/104303404322886156 (literal)

Alternative label

• Sara Deola, Samantha Scaramuzza, Roberto Sciarretta Birolo, Nicole Carballido-Perrig, Francesca Ficara, Cristina Mocchetti, Jonathan Dando, José M. Carballido, Claudio Bordignon, Maria Grazia Roncarolo, Marco Bregni, and Alessandro Aiuti. (2004)
  Mobilized blood CD34+ cells transduced and selected with a clinically applicable protocol reconstitute lymphopoiesis in SCID-Hu mice.
  in Human gene therapy
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Sara Deola, Samantha Scaramuzza, Roberto Sciarretta Birolo, Nicole Carballido-Perrig, Francesca Ficara, Cristina Mocchetti, Jonathan Dando, José M. Carballido, Claudio Bordignon, Maria Grazia Roncarolo, Marco Bregni, and Alessandro Aiuti. (literal)

Pagina inizio

• 305 (literal)

Pagina fine

• 311 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 15 (literal)

Rivista

• Human gene therapy (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 3 (literal)

Note

• ISI Web of Science (WOS) (literal)
• Scopus (literal)
• Google Scholar (literal)
• PubMe (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• San Raffaele Telethon Institute for Gene Therapy (HSR-TIGET), 20132 Milan, Italy Novartis Research Institute, A-1235 Vienna, Austria Hematology and BMT Unit, Scientific Institute H.S. Raffaele, 20132 Milan, Italy (literal)

Titolo

• Mobilized blood CD34+ cells transduced and selected with a clinically applicable protocol reconstitute lymphopoiesis in SCID-Hu mice. (literal)

Abstract

• We developed a clinically applicable gene transfer procedure into mobilized peripheral blood (MPB) CD34+ hematopoietic progenitor cells, based on single viral exposure and selection of engineered cells. CD34+ cells were transduced with a retroviral vector carrying the truncated form of the nerve growth factor receptor (?NGFR) marker gene, and immunoselected for ?NGFR expression. Optimal time and procedure for viral exposure, length of culture, and transgene expression of MPB CD34+ cells were determined using in vitro assays. The multipotent capacity of MPB CD34+-transduced cells was demonstrated in the SCID-hu bone/liver/thymus mouse model. Transduced ?NGFR+ cells retained 50% of long-term culture-colony forming cells (LTC-CFC) compared to unmanipulated CD34+ cells. In SCID-hu mice, 52% of CD45+ cells, 27% of CD34+ cells, 49% of B cells, and more than 50% of T cells were derived from transplanted CD34+/?NGFR+ cells. Furthermore, transplantation of purified transduced cells greatly reduced the competition with untransduced progenitors occurring in unselected grafts. These data demonstrate that MPB CD34+ cells, transduced with a single viral exposure and selected by transgene expression, retain multilineage reconstitution capacity and remarkable transgene expression. (literal)

Prodotto di

• Istituto di Ricerca Genetica e Biomedica (IRGB) (Istituto)

Autore CNR

• FRANCESCA FICARA (Persona)

Incoming links:

Prodotto

• Istituto di Ricerca Genetica e Biomedica (IRGB) (Istituto)

Autore CNR di

• FRANCESCA FICARA (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Human gene therapy (Rivista)