The serotonin receptor 7 promotes neurite outgrowth via ERK and Cdk5 signaling pathways. (Articolo in rivista)

Type
Label
  • The serotonin receptor 7 promotes neurite outgrowth via ERK and Cdk5 signaling pathways. (Articolo in rivista) (literal)
Anno
  • 2013-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1016/j.neuropharm.2012.10.026 (literal)
Alternative label
  • Speranza L, Chambery A, Di Domenico M, Crispino M, Severino V, Volpicelli F, Leopoldo M, Bellenchi GC, di Porzio U, Perrone-Capano C. (2013)
    The serotonin receptor 7 promotes neurite outgrowth via ERK and Cdk5 signaling pathways.
    in Neuropharmacology
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Speranza L, Chambery A, Di Domenico M, Crispino M, Severino V, Volpicelli F, Leopoldo M, Bellenchi GC, di Porzio U, Perrone-Capano C. (literal)
Pagina inizio
  • 155 (literal)
Pagina fine
  • 167 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 67 (literal)
Rivista
Note
  • PubMe (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Department of Biological Sciences, University of Naples Federico II, Section of Physiology, Via Mezzocannone 8, 80134 Naples, Italy Institute of Genetics and Biophysics \"Adriano Buzzati Traverso\", CNR, Naples, Italy Department of Life Sciences, Second University of Naples, Italy Department of Experimental Pharmacology, University of Naples Federico II, Naples, Italy Department of Medicinal Chemistry, University of Bari, Italy (literal)
Titolo
  • The serotonin receptor 7 promotes neurite outgrowth via ERK and Cdk5 signaling pathways. (literal)
Abstract
  • Serotonergic neurotransmission is mediated by at least 14 subtypes of 5-HT receptors. Among these, the CNS serotonin receptor 7 (5-HTR7) is involved in diverse physiological processes. Here we show that treatment of murine striatal and cortical neuronal cultures with 5-HTR7 agonists (8-OH-DPAT and LP-211) significantly enhances neurite outgrowth. This effect is abolished by the selective 5- HTR7 antagonist SB-269970, by the ERK inhibitor U0126, by the cyclin-dependent kinase 5 (Cdk5) inhibitor roscovitine, as well as by cycloheximide, an inhibitor of protein synthesis. These data indicate that 5-HTR7 activation stimulates extensive neurite elongation in CNS primary cultures, subserved by ERK and Cdk5 activation, and de novo protein synthesis. Two-dimensional (2D) gel electrophoresis coupled to Western blot analyses reveals both qualitative and quantitative expression changes in selected cytoskeletal proteins, following treatment of striatal primary cultures with LP-211. In particular, the 34 kDa isoform of MAP1B is selectively expressed in stimulated cultures, consistent with a role of this protein in tubulin polymerization and neurite elongation. In summary, our results show that agonist-dependent activation of the endogenous 5- HTR7 in CNS neuronal primary cultures stimulates ERK- and Cdk5-dependent neurite outgrowth, sustained by modifications of cytoskeletal proteins. These data support the hypothesis that the 5-HTR7 might play a crucial role in shaping neuronal morphology and behaviorally relevant neuronal networks, paving the way to new approaches able to modulate CNS connectivity. (literal)
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