http://www.cnr.it/ontology/cnr/individuo/prodotto/ID19165

Prolactin and insulin ultradian secretion and adipose tissue lipoprotein lipase expression in severely obese women after bariatric surgery (Articolo in rivista)

Type

Prodotto della ricerca (Classe)
Articolo in rivista (Classe)

Label

Prolactin and insulin ultradian secretion and adipose tissue lipoprotein lipase expression in severely obese women after bariatric surgery (Articolo in rivista) (literal)

Anno

2008-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

10.1038/oby.2008.297 (literal)

Alternative label

Mingrone, G 1; Manco, M 2; Iaconelli, A 1; Gniuli, D 1; Bracaglia, R 3; Leccesi, L 1; Calvani, M 1; Nolfe, G 4; Basus, S 5; Berria, R 5 (2008)
Prolactin and insulin ultradian secretion and adipose tissue lipoprotein lipase expression in severely obese women after bariatric surgery
in Obesity (Silver Spring, Md.)
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Mingrone, G 1; Manco, M 2; Iaconelli, A 1; Gniuli, D 1; Bracaglia, R 3; Leccesi, L 1; Calvani, M 1; Nolfe, G 4; Basus, S 5; Berria, R 5 (literal)

Pagina inizio

1831 (literal)

Pagina fine

1837 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

16 (literal)

Rivista

Obesity (Rivista)

Note

PubMe (literal)
ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

1. Univ Cattolica Sacro Cuore, Dept Internal Med, Rome, Italy 2. Bambino Gesu Hosp & Res Inst, Liver Unit, Rome, Italy 3. Univ Cattolica Sacro Cuore, Dept Surg, Rome, Italy 4. CNR, Inst Cybernet E Caianiello, Pozzuoli, Italy 5. Case Western Reserve Univ, Metrohlth Med Ctr, Dept Obstet & Gynecol, Cleveland, OH USA (literal)

Titolo

Prolactin and insulin ultradian secretion and adipose tissue lipoprotein lipase expression in severely obese women after bariatric surgery (literal)

Abstract

Background: Hyperprolactinemia is associated with obesity. Furthermore, in human adipose tissue cultured in vitro, prolactin (PRL) inhibited lipoprotein lipase (LPL) activity via functional PRL receptors. Objective: Tostudy PRL and insulin ultradian rhythm and subcutaneous adipose tissue LPL mRNA and protein expressions in severely obese women before and after malabsorptive bariatric surgery. Methods and Procedures: Seven severely obese, fertile women were studied twice, once before and the second time 1 year after bilio-pancreatic diversion (BPD), when the weight was stable for at least 3 months. Metabolizable energy intake and 24-h energy expenditure (EE) were measured. Fourier and PULSEFIT analyses were applied to 24-h hormonal time-series to study daily fluctuations and hormonal clearance. Insulin sensitivity was assessed by euglycemic-hyperinsulinemic clamp. Quantitative-competitive reverse transcriptase-PCR and western blot analysis were used to measure LPL gene expression. Results: Spontaneous 24-h PRL secretion was significantly reduced after BPD (mean-daily release, 128.4 ± 28.1 ?g/l vs. 67.2 ± 9.2 ?g/l distribution volume (Vd/l·24 h), P = 0.02); insulin secretion also was significantly reduced (499.9 ± 204.0 ?g/Vd/l·24 h vs. 85.6 ± 21.0 ?g/Vd/l·24 h, P = 0.0001). Metabolizable energy/kgFFM did not change significantly after BPD. Twenty-four-hour EE, but not 24-h EE/FFM, was significantly decreased after BPD (P < 0.05). Insulin sensitivity significantly (P < 0.0001) increased after BPD from 21.41 ± 1.92 to 68.62 ± 5.03 ?mol/kgFFM/min. LPL mRNA concentration (from 42.63 ± 4.21% to 19.00 ± 2.74% of cyclophilin mRNA, P = 0.001) as well as LPL protein level (from 8.94 ± 2.73 to 3.16 ± 1.05 as ratios of protein of interest vs. housekeeping protein, P = 0.038) significantly decreased after BPD. The major determinant of PRL secretion was insulin secretion, whereas the best predictors of LPL expression were insulin and PRL secretion rates. Discussion: The restriction of lipid metabolizable energy rather than weight loss seems to be responsible for both reduction in PRL circulating levels and normalization of its secretion rhythm after bariatric surgery. Furthermore, the reduced adipose tissue LPL expression, being significantly correlated with the decrease in insulin and PRL, suggests a role of hyperinsulinemia and hyperprolactinemia in inducing and sustaining obesity. (literal)

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