Voluntary alcohol drinking enhances proopiomelanocortin (POMC) gene expression in nucleus accumbens shell and hypothalamus of Sardinian alcohol-preferring rats. (Articolo in rivista)

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  • Voluntary alcohol drinking enhances proopiomelanocortin (POMC) gene expression in nucleus accumbens shell and hypothalamus of Sardinian alcohol-preferring rats. (Articolo in rivista) (literal)
Anno
  • 2013-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1111/j.1530-0277.2012.01867.x (literal)
Alternative label
  • Zhou Y., Colombo G., Niikura K., Carai M.A.M., Femenía T., García-Gutiérrez M.S., Manzanares J., Ho A., Gessa G.L., Kreek M.J. (2013)
    Voluntary alcohol drinking enhances proopiomelanocortin (POMC) gene expression in nucleus accumbens shell and hypothalamus of Sardinian alcohol-preferring rats.
    in Alcoholism, clinical and experimental research
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Zhou Y., Colombo G., Niikura K., Carai M.A.M., Femenía T., García-Gutiérrez M.S., Manzanares J., Ho A., Gessa G.L., Kreek M.J. (literal)
Pagina inizio
  • E131 (literal)
Pagina fine
  • E140 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 37 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
  • 10 (literal)
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  • S1 (literal)
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  • Laboratory of the Biology of Addictive Diseases, The Rockefeller University, New York, NY, USA; Neuroscience Institute, National Research Council of Italy, Section of Cagliari, Monserrato (CA), Italy; Instituto de Neurociencias, Universidad Miguel Hernández-CSIC, San Juan de Alicante, Alicante, Spain (literal)
Titolo
  • Voluntary alcohol drinking enhances proopiomelanocortin (POMC) gene expression in nucleus accumbens shell and hypothalamus of Sardinian alcohol-preferring rats. (literal)
Abstract
  • Background: Evidence obtained in humans and rodents indicates that beta-endorphin [encoded by the proopiomelanocortin (POMC) gene] is critical in regulation of alcohol drinking behavior. However, the alcohol effect on POMC gene expression has not been studied in rodent mesolimbic regions, such as the nucleus accumbens (NAc). Methods: In this study, we first utilized POMC-EGFP transgenic mice to visualize POMC neurons, and found that POMC-EGFP cells were modestly distributed throughout NAc shell and core, in addition to hypothalamic arcuate nucleus. POMC mRNA expression in NAc of mice and rats was confirmed using RT-PCR and solution hybridization assays. We then investigated whether there are genetically determined differences in basal mRNA levels of POMC and mu opioid receptor (MOP-r) between selectively bred Sardinian alcohol-preferring (sP) and -nonpreferring (sNP) rats, and if these mRNA levels are altered in sP after alcohol drinking (10%, unlimited access) for 17 days. Results: Alcohol-naive sP rats had higher basal POMC mRNA levels than sNP only in hypothalamus. Alcohol drinking increased POMC mRNA levels in both NAc shell (100%) and hypothalamus (50%) of sP. Although sP had lower basal levels of MOP-r mRNA and GTP?S binding in NAc shell than sNP rats, voluntary alcohol consumption had no effect on MOP-r mRNA levels in NAc shell. Conclusion: Our results define the distribution of POMC-expressing neurons in NAc of mice and rats. Higher POMC expression at basal levels in alcohol-preferring sP rats(genetically determined), along with increases after drinking (alcohol-induced) in NAc shell and hypothalamus, suggest that the POMC systems play a role in high alcohol preference and consumption. (literal)
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