A new cell-selective three-dimensional microincubator based on silicon photonic crystals. (Articolo in rivista)

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Label
  • A new cell-selective three-dimensional microincubator based on silicon photonic crystals. (Articolo in rivista) (literal)
Anno
  • 2012-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1371/journal.pone.0048556 (literal)
Alternative label
  • Carpignano F, Silva G, Surdo S, Leva V, Montecucco A, Aredia F, Scovassi AI, Merlo S, Barillaro G, Mazzini G. (2012)
    A new cell-selective three-dimensional microincubator based on silicon photonic crystals.
    in PloS one
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Carpignano F, Silva G, Surdo S, Leva V, Montecucco A, Aredia F, Scovassi AI, Merlo S, Barillaro G, Mazzini G. (literal)
Pagina inizio
  • e48556 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 7 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 11 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Dipartimento di Ingegneria Industriale e dell'Informazione, Università di Pavia, Pavia, Italy Dipartimento di Ingegneria dell'Informazione, Elettronica, Informatica, Telecomunicazioni, Università di Pisa, Pisa, Italy IGM-CNR, Pavia, Italy Dipartimento di Biologia e Biotecnologie \"L. Spallanzani\", Università di Pavia, Pavia, Italy (literal)
Titolo
  • A new cell-selective three-dimensional microincubator based on silicon photonic crystals. (literal)
Abstract
  • In this work, we show that vertical, high aspect-ratio (HAR) photonic crystals (PhCs), consisting of periodic arrays of 5 mm wide gaps with depth of 50 mm separated by 3 mm thick silicon walls, fabricated by electrochemical micromachining, can be used as three-dimensional microincubators, allowing cell lines to be selectively grown into the gaps. Silicon micromachined dice incorporating regions with different surface profiles, namely flat silicon and deeply etched PhC, were used as microincubators for culturing adherent cell lines with different morphology and adhesion properties. We extensively investigated and compared the proliferative behavior on HAR PhCs of eight human cell models, with different origins, such as the epithelial (SW613-B3; HeLa; SW480; HCT116; HT29) and the mesenchymal (MRC-5V1; CF; HT1080). We also verified the contribution of cell sedimentation into the silicon gaps. Fluorescence microscopy analysis highlights that only cell lines that exhibit, in the tested culture condition, the behavior typical of the mesenchymal phenotype are able to penetrate into the gaps of the PhC, extending their body deeply in the narrow gaps between adjacent silicon walls, and to grow adherent to the vertical surfaces of silicon. Results reported in this work, confirmed in various experiments, strongly support our statement that such three-dimensional microstructures have selection capabilities with regard to the cell lines that can actively populate the narrow gaps. Cells with a mesenchymal phenotype could be exploited in the next future as bioreceptors, in combination with HAR PhC optical transducers, e.g., for label-free optical detection of cellular activities involving changes in cell adhesion and/or morphology (e.g., apoptosis) in a three-dimensional microenvironment. (literal)
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