Expression of rat diamine oxidase in Escherichia coli (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Expression of rat diamine oxidase in Escherichia coli (Articolo in rivista) (literal)

Anno

• 2012-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/j.molcatb.2012.06.014 (literal)

Alternative label

• Rosini, Elena; Nossa, Serena; Valentino, Mattia; D'Arrigo, Paola; Marinesco, Stephane; Pollegioni, Loredano (2012)
  Expression of rat diamine oxidase in Escherichia coli
  in Journal of molecular catalysis. B, Enzymatic (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Rosini, Elena; Nossa, Serena; Valentino, Mattia; D'Arrigo, Paola; Marinesco, Stephane; Pollegioni, Loredano (literal)

Pagina inizio

• 115 (literal)

Pagina fine

• 120 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 82 (literal)

Rivista

• Journal of molecular catalysis. B, Enzymatic (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 6 (literal)

Note

• ISI Web of Science (WoS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• University of Insubria; ICRM CNR Milano; CNR Ist Chim Riconascimento Mol ICRM; Polytechnic University of Milan; Centre National de la Recherche Scientifique (CNRS); PRES University of Lyon (literal)

Titolo

• Expression of rat diamine oxidase in Escherichia coli (literal)

Abstract

• In this work, a reliable protocol was designed to rapidly express and purify a rat diamine oxidase in Escherichia coli as a useful alternative to enzyme isolated from animal sources. The cDNA encoding for rat diamine oxidase was overexpressed in an Origami2(DE3) E. coli strain and, by employing a rapid purification protocol in which the hexahistidine tag was added at the C-terminal end of the enzyme, the recombinant oxidase could be purified in a single step on a Ni-NTA column at >95% purity. The enzyme was active but was largely produced in an immature quinone form: Cu2+ ions stimulated further activation/maturation. This expression and purification procedure offers an easy and rapid means of producing recombinant rat diamine oxidase from an animal-free source and represents a useful tool to boost biotechnological application of this enzyme. (C) 2012 Elsevier B.V. All rights reserved. (literal)

Prodotto di

• Applicazioni sintetiche della biocatalisi (PM.P03.009.002) (Modulo)
• Istituto di chimica del riconoscimento molecolare (ICRM) (Istituto)

Autore CNR

• MATTIA VALENTINO (Unità di personale interno)

Insieme di parole chiave

• Keywords of "Expression of rat diamine oxidase in Escherichia coli" (Insieme di parole chiave)

Incoming links:

Prodotto

• Istituto di chimica del riconoscimento molecolare (ICRM) (Istituto)
• Applicazioni sintetiche della biocatalisi (PM.P03.009.002) (Modulo)

Autore CNR di

• MATTIA VALENTINO (Unità di personale interno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Journal of molecular catalysis. B, Enzymatic (Rivista)

Insieme di parole chiave di

• Keywords of "Expression of rat diamine oxidase in Escherichia coli" (Insieme di parole chiave)