http://www.cnr.it/ontology/cnr/individuo/prodotto/ID187173
Cattle breed traceability in meat and cheese using dna polymorphisms detected by the snapshot method (Articolo in rivista)
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- Label
- Cattle breed traceability in meat and cheese using dna polymorphisms detected by the snapshot method (Articolo in rivista) (literal)
- Anno
- 2009-01-01T00:00:00+01:00 (literal)
- Alternative label
D'Andrea, M (D'Andrea, M.)1; Merigioli, A (Merigioli, A.)2; Scarano, MT (Scarano, M. -T.)1,3; Pilla, F (Pilla, F.)1 (2009)
Cattle breed traceability in meat and cheese using dna polymorphisms detected by the snapshot method
in Italian journal of food sciences (Testo stamp.)
(literal)
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- D'Andrea, M (D'Andrea, M.)1; Merigioli, A (Merigioli, A.)2; Scarano, MT (Scarano, M. -T.)1,3; Pilla, F (Pilla, F.)1 (literal)
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- ISI Web of Science (WOS) (literal)
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- 1. Univ Molise, Dipartimento Sci Anim Vegetali & Ambiente, Molise, Italy
2. Parco Sci & Tecnol Molise Moliseinnovaz Scarl, Campobasso, Italy
3. CNR, Sez Palermo, Ist Genet Vegetale, I-00185 Rome, Italy (literal)
- Titolo
- Cattle breed traceability in meat and cheese using dna polymorphisms detected by the snapshot method (literal)
- Abstract
- Breed identification of animal products is a fundamental tool used to control food quality, add value to regional products and exploit local breeds as well as prevent food adulteration. Myostatin (MSTN) and Melanocortin I Receptor (MC1R) are ideal candidate genes for molecular breed traceability of cattle because of their breed-specific variability. A novel method based on primer extension (SNaPshot) was successfully used for the MSTN and MC1R genotyping of eight bovine breeds. A total of 114 samples from hair bulb and cheese were analyzed. The results obtained allowed the breeds to be identified and results were confirmed by PCR-RFLP and DNA sequencing. The proposed SNaPshot method provides rapid throughput analysis from highly fragmented DNA of processed foods from animals, such as cheese (literal)
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