A new respirometric endpoint-based biosensor to assess the relative toxicity of chemicals on immobilized human cells (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• A new respirometric endpoint-based biosensor to assess the relative toxicity of chemicals on immobilized human cells (Articolo in rivista) (literal)

Anno

• 2009-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/j.ecoenv.2008.02.011 (literal)

Alternative label

• Roberto Dragone; Chiara Frazzoli; Claudio Grappelli; Luigi Campanella (2009)
  A new respirometric endpoint-based biosensor to assess the relative toxicity of chemicals on immobilized human cells
  in Ecotoxicology and environmental safety
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Roberto Dragone; Chiara Frazzoli; Claudio Grappelli; Luigi Campanella (literal)

Pagina inizio

• 273 (literal)

Pagina fine

• 279 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 72 (literal)

Rivista

• Ecotoxicology and environmental safety (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 7 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Roberto Dragone: Institute for Complex Systems, Consiglio Nazionale delle Ricerche, P.le Aldo Moro 5, 00185 Rome, Italy Chiara Frazzoli: Department of Food Safety and Veterinary Public Health and WHO/FAO Collaborating Centre for Veterinary Public Health, Istituto Superiore di Sanita`, V.le Regina Elena 299, 00161 Rome, Italy Claudio Grappelli: Department of Genetics and Molecular Biology, University of Rome ''LaSapienza'', P.le Aldo Moro 5, 00185 Rome, Italy Luigi Campanella: Department of Chemistry, University ''LaSapienza'', P.le Aldo Moro 5, 00185 Rome, Italy (literal)

Titolo

• A new respirometric endpoint-based biosensor to assess the relative toxicity of chemicals on immobilized human cells (literal)

Abstract

• Several functional and biochemical parameters have been proposed as biomarkers of effect of environmental pollutants. A rapid biosensor working with immobilizer human U-937cells was developed and applied to environmentally relevant chemicals with different structures and toxicological pathways, i.e.benzalkonium chloride, clofibri cacid, diclofenac, mercury nitrate, ofloxacin, and sodium dodecyl sulphate. Respiration of cells was relied upon as a comprehensive biochemical effect for screening purposes. Analytical parameter(?ppmO2) and toxicological index (respiratory inhibition, ?%) measured after1h of exposure were utilized for dose-response relationship study. Results (toxicity rating scales based on ?50% and steepness) were compared with those obtained by the same approach previously optimized on Saccharomyces cerevisiae. The toxicity rating scale obtained by the biomarker based on human mitochondrial and cell metabolic activities compared well with previous scale obtained on yeast cells and with available in-vivo acute toxicity indexes; respiration was confirmed as toxicological endpoint reliably measurable by the biosensor. (literal)

Prodotto di

• Materiali inorganici complessi per catalisi eterogenea. (Bio)sensori. (MD.P02.005.002) (Modulo)

Autore CNR

• ROBERTO DRAGONE (Persona)

Insieme di parole chiave

• Keywords of "A new respirometric endpoint-based biosensor to assess the relative toxicity of chemicals on immobilized human cells" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• ROBERTO DRAGONE (Persona)

Prodotto

• Materiali inorganici complessi per catalisi eterogenea. (Bio)sensori. (MD.P02.005.002) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Ecotoxicology and environmental safety (Rivista)

Insieme di parole chiave di

• Keywords of "A new respirometric endpoint-based biosensor to assess the relative toxicity of chemicals on immobilized human cells" (Insieme di parole chiave)