http://www.cnr.it/ontology/cnr/individuo/prodotto/ID185063

Evidence for a role of inducible nitric oxide synthase in gastric relaxation of mdx mice (Articolo in rivista)

Type

Prodotto della ricerca (Classe)
Articolo in rivista (Classe)

Label

Evidence for a role of inducible nitric oxide synthase in gastric relaxation of mdx mice (Articolo in rivista) (literal)

Anno

2006-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

10.1111/j.1365-2982.2006.00782.x (literal)

Alternative label

Mule, F; Zizzo, MG; Amato, A; Feo, S; Serio, R (2006)
Evidence for a role of inducible nitric oxide synthase in gastric relaxation of mdx mice
in Neurogastroenterology and motility (Print); Blackwell publishing, Oxford (Regno Unito)
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Mule, F; Zizzo, MG; Amato, A; Feo, S; Serio, R (literal)

Pagina inizio

446 (literal)

Pagina fine

454 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

18 (literal)

Rivista

Neurogastroenterology and motility (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

9 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

6 (literal)

Note

ISI Web of Science (WOS) (literal)
PubMe (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

Dipartimento Biologia Cellulare e dello Sviluppo, Università di Palermo, Italy Dipartimento Oncologia Sperimentale e Applicazioni Cliniche, Università di Palermo, Italy (literal)

Titolo

Evidence for a role of inducible nitric oxide synthase in gastric relaxation of mdx mice (literal)

Abstract

Alterations of gastric mechanical activity have been reported in mdx mouse, animal model for Duchenne muscular dystrophy. This study examined if alterations in the vasoactive intestinal polypeptide (VIP) system are present in mdx stomach. Gastric mechanical activity was recorded in vitro as changes of endoluminal pressure and neurally or pharmacologically evoked relaxations were analysed in mdx vs normal stomach. Reverse-transcription polymerase chain reaction was used to detect inducible nitric oxide synthase (iNOS) expression. Relaxations to sodium nitroprusside in mdx stomach showed no difference in comparison with normal preparations. In normal stomach, VIP produced relaxation, which was reduced by VIP6-28, antagonist of VIP receptors, but was not modified by N-omega-nitro-L-arginine methyl ester (L-NAME), 1-H-oxodiazol-[1,2,4]-[4,3-a]quinoxaline-1-one(ODQ) or by N-(3-(aminomethyl)-benzyl)acetamidine (1400W) and aminoguanidine, inhibitors of iNOS. In contrast, in mdx stomach VIP responses were antagonized not only by VIP6-28, but also by L-NAME, ODQ, 1400W or aminoguanidine. In normal stomach, the slow relaxation evoked by stimulation at high frequency was reduced by VIP6-28, but it was unaffected by 1400W or aminoguanidine. In mdx stomach, it was reduced by VIP6-28 or 1400W, which did not show additive effects. iNOS mRNA was expressed only in mdx stomach. The results suggest that in mdx gastric preparations, iNOS is functionally expressed, being involved in the slow relaxation induced by VIP. (literal)

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