http://www.cnr.it/ontology/cnr/individuo/prodotto/ID184343

Pitfalls in determining size homoplasy of microsatellite loci (Articolo in rivista)

Type

Label

Pitfalls in determining size homoplasy of microsatellite loci (Articolo in rivista) (literal)

Anno

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

Alternative label

Liepelt S; Kuhlenkamp, V; Anzidei, M; Vendramin, GG; Ziegenhagen, B; (2001)
Pitfalls in determining size homoplasy of microsatellite loci
in Molecular ecology notes (Print)
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Pagina inizio

Pagina fine

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

http://onlinelibrary.wiley.com/doi/10.1046/j.1471-8278.2001.00085.x/pdf (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

Rivista

Note

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

Federal Research Centre for Forestry and Forest Products, Institute for Forest Genetics and Forest Tree Breeding, Sieker Landstrasse 2, 22927 Grosshansdorf, Germany, C.N.R, Institute of Forest Tree Breeding, Via Atto Vannucci, 13, I-50134 Firenze, Italy (literal)

Titolo

Abstract

Size homoplasy of microsatellite loci can be detected by single-strand conformation polymorphism analysis (SSCP) or sequence analysis. The present note demonstrates pitfalls of sequencing microsatellite loci. A complex chloroplast microsatellite locus was polymerase chain reaction (PCR) amplified from 33 Abies alba individuals, and the products cloned and sequenced. After 2-7 clones per individual had been sequenced and aligned, differences in repeat numbers occurred among clones of the same individual. It was concluded that the DNA polymerase slippage during PCR most probably caused this variation. A solution specific to the analysed complex locus was found by splitting it into two new loci. (literal)

Prodotto di