Smart surfaces for pH controlled cell staining (Articolo in rivista)

Type
Label
  • Smart surfaces for pH controlled cell staining (Articolo in rivista) (literal)
Anno
  • 2009-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1039/b914277k (literal)
Alternative label
  • Simona Argentiere , Laura Blasi , Giuseppe Ciccarella , Antonella Cazzato , Giovanna Barbarella , Roberto Cingolani , Giuseppe Gigli (2009)
    Smart surfaces for pH controlled cell staining
    in Soft matter (Online)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Simona Argentiere , Laura Blasi , Giuseppe Ciccarella , Antonella Cazzato , Giovanna Barbarella , Roberto Cingolani , Giuseppe Gigli (literal)
Pagina inizio
  • 4101 (literal)
Pagina fine
  • 4103 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 5 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • 1. CNR INFM, Natl Nanotechnol Labs, Lecce, Italy (Simona Argentiere , Laura Blasi , Giuseppe Ciccarella , Roberto Cingolani , Giuseppe Gigli ) 2. ISOF, CNR, Area Ric, I-40129 Bologna, Italy (Antonella Cazzato , Giovanna Barbarella ,) (literal)
Titolo
  • Smart surfaces for pH controlled cell staining (literal)
Abstract
  • In this work, novel smart surfaces for in situ cell staining were realized by covalent attachment of pH-responsive microgels on platforms dedicated to microfluidics for lab-on-chip. The poly(methacrylic acid) microgels were firstly synthesized in solution and then covalently immobilized on a glass surface. As they preserve their pH-sensitive nature after the covalent immobilization, microgels were loaded with an oligothiophene-conjugated anti-human CD4 monoclonal antibody, and finally incubated with a Jurkat T-cell suspension. The physiological pH of the extracellular environment induced the pH-triggered release of the labeled antiCD4 antibodies and the selective staining of the CD4-positive subpopulations within the Jurkat cell suspension. The realization of this type of smart surface for the encapsulation of specific monoclonal antibodies and their release in an on-demand way should have an enormous potential in developing fully integrated platforms for cell analysis. (literal)
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