Biosynthetic Oligosaccharide Libraries for Identification of Protein-binding Heparan Sulfate Motifs. EXPLORING THE STRUCTURAL DIVERSITY BY SCREENING FOR FIBROBLAST GROWTH FACTOR (FGF) 1 AND FGF2 BINDING (Articolo in rivista)

Type
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  • Biosynthetic Oligosaccharide Libraries for Identification of Protein-binding Heparan Sulfate Motifs. EXPLORING THE STRUCTURAL DIVERSITY BY SCREENING FOR FIBROBLAST GROWTH FACTOR (FGF) 1 AND FGF2 BINDING (Articolo in rivista) (literal)
Anno
  • 2002-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1074/jbc.M203404200 (literal)
Alternative label
  • P.Jemth, J.Kreuger, M. Kusche-Gullberg, L.Sturiale, G.Gimenez-Gallego, U.Lindahl (2002)
    Biosynthetic Oligosaccharide Libraries for Identification of Protein-binding Heparan Sulfate Motifs. EXPLORING THE STRUCTURAL DIVERSITY BY SCREENING FOR FIBROBLAST GROWTH FACTOR (FGF) 1 AND FGF2 BINDING
    in Journal of biological chemistry (Online); AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, BETHESDA (Stati Uniti d'America)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • P.Jemth, J.Kreuger, M. Kusche-Gullberg, L.Sturiale, G.Gimenez-Gallego, U.Lindahl (literal)
Pagina inizio
  • 30567 (literal)
Pagina fine
  • 30573 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni
  • Impact factor: 6.696 Times cited: 60 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 277 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
  • 7 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 34 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto
  • Questo lavoro, frutto di una collaborazione con l’Istituto di Ricerche Chimiche e Biochimiche G. Ronzoni di Milano e il Dept. of Medical Biochemistry and Microbiology -Uppsala University, ha consentito di generare “in vitro” una serie di epitopi oligosaccaridici in grado di mimare l’attività biologica dell’Eparan solfato, biopolimero naturale di struttura eterogenea che si trova coinvolto in numerosi processi biologici come l’angiogenesi, la proliferazione e la differenziazione cellulare. Tali librerie oligosacaridiche, ottenute a partire dall’Eparina per depolimerizzazione chimica e successiva modificazione enzimatica, sono state ottenute selezionando cromatograficamente il pool di oligomeri ottenuto in base al grado di affinità con molecole biologicamente attive quali i fattori di crescita dei fibroblasti (Fibroblast Growth Factors: FGF-1, FGF-2). La caratterizzazione delle sequenze oligosaccaridiche ad alta affinità è stata ottenuta con metodi enzimatici e tramite spettrometria di massa MALDI –TOF. (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Uni Uppsala; Istituto Ronzoni Milano - CNR; CIB Madrid. (literal)
Titolo
  • Biosynthetic Oligosaccharide Libraries for Identification of Protein-binding Heparan Sulfate Motifs. EXPLORING THE STRUCTURAL DIVERSITY BY SCREENING FOR FIBROBLAST GROWTH FACTOR (FGF) 1 AND FGF2 BINDING (literal)
Abstract
  • Heparan sulfate is crucial for vital reactions in the body because of its ability to bind various proteins. The identification of protein-binding heparan sulfate sequences is essential to our understanding of heparan sulfate biology and raises the possibility to develop drugs against diseases such as cancer and inflammatory conditions. We present proof-of-principle that in vitro generated heparan sulfate oligosaccharide libraries can be used to explore interactions between heparan sulfate and proteins, and that the libraries expand the available heparan sulfate sequence space. Oligosaccharide libraries mimicking highly 6-O-sulfated domains of heparan sulfate were constructed by enzymatic O-sulfation of O-desulfated, end-group 3H-labeled heparin octasaccharides. Acceptor oligosaccharides that were 6-O-desulfated but only partially 2-O-desulfated yielded oligosaccharide arrays with increased ratio of iduronyl 2-O-sulfate/glucosaminyl 6-O-sulfate. The products were probed by affinity chromatography on immobilized growth factors, fibroblast growth factor-1 (FGF1) and FGF2, followed by sequence analysis of trapped oligosaccharides. An N-sulfated octasaccharide, devoid of 2-O-sulfate but with three 6-O-sulfate groups, was unexpectedly found to bind FGF1 as well as FGF2 at physiological ionic strength. However, a single 2-O-sulfate group in the absence of 6-O-sulfation gave higher affinity for FGF2. FGF1 binding was also augmented by 2-O-sulfation, preferentially in combination with an adjacent upstream 6-O-sulfate group. These results demonstrate the potential of the enzymatically generated oligosaccharide libraries. (literal)
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