Structural and functional characterization of FoF1-ATP synthase on the extracellular surface of rat hepatocytes (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Structural and functional characterization of FoF1-ATP synthase on the extracellular surface of rat hepatocytes (Articolo in rivista) (literal)

Anno

• 2008-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/j.bbabio.2008.08.003 (literal)

Alternative label

• Mangiullo R; Gnoni A; Leone A; Gnoni GV; Papa S; Zanotti F; (2008)
  Structural and functional characterization of FoF1-ATP synthase on the extracellular surface of rat hepatocytes
  in Biochimica et biophysica acta. Bioenergetics; ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS, AMSTERDAM (Paesi Bassi)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Mangiullo R; Gnoni A; Leone A; Gnoni GV; Papa S; Zanotti F; (literal)

Pagina inizio

• 1326 (literal)

Pagina fine

• 1335 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

• http://www.sciencedirect.com/science/article/pii/S0005272808006555 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 1777 (literal)

Rivista

• Biochimica et biophysica acta. Bioenergetics (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 10 (literal)

Note

• Google Scholar (literal)
• PubMe (literal)
• Scopu (literal)
• Elsevier (literal)
• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Department of Medical Biochemistry, Biology and Physics, University of Bari, Italy; Institute of Sciences of Food Production, CNR, ISPA, Lecce, Italy; Laboratory of Biochemistry, Department of Biological and Environmental Sciences and Technology, University of Salento, Lecce, Italy; Institute of Biomembranes and Bioenergetics, CNR, Bari, Italy; (literal)

Titolo

• Structural and functional characterization of FoF1-ATP synthase on the extracellular surface of rat hepatocytes (literal)

Abstract

• Extracellular ATP formation from ADP and inorganic phosphate, attributed to the activity of a cell surface ATP synthase, has so far only been reported in cultures of some proliferating and tumoral cell lines. We now provide evidence showing the presence of a functionally active ecto-FoF1-ATP synthase on the plasma membrane of normal tissue cells, i.e. isolated rat hepatocytes. Both confocal microscopy and flow cytometry analysis show the presence of subunits of F1 (OE±/OE<= and OE>=) and Fo (FoI-PVP(b) and OSCP) moieties of ATP synthase at the surface of rat hepatocytes. This finding is confirmed by immunoblotting analysis of the hepatocyte plasma membrane fraction. The presence of the inhibitor protein IF1 is also detected on the hepatocyte surface. Activity assays show that the ectopic-ATP synthase can work both in the direction of ATP synthesis and hydrolysis. A proton translocation assay shows that both these mechanisms are accompanied by a transient flux of H+ and are inhibited by F1 and Fo-targeting inhibitors. We hypothesise that ecto-FoF1-ATP synthase may control the extracellular ADP/ATP ratio, thus contributing to intracellular pH homeostasis. (literal)
• Impact Factor: 4.829 Cited: 48 (literal)

Editore

• ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS (Editore)

Prodotto di

• Ottenimento di nuovi prodotti per la salute, l'alimentazione e l'industria mediante interventi genetici e biotecnologici nelle piante (AG.P01.006.001) (Modulo)
• Istituto di scienze delle produzioni alimentari (ISPA) (Istituto)

Autore CNR

• ANTONELLA LEONE (Unità di personale interno)

Incoming links:

Autore CNR di

• ANTONELLA LEONE (Unità di personale interno)

Prodotto

• Istituto di scienze delle produzioni alimentari (ISPA) (Istituto)
• Ottenimento di nuovi prodotti per la salute, l'alimentazione e l'industria mediante interventi genetici e biotecnologici nelle piante (AG.P01.006.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Biochimica et biophysica acta. Bioenergetics (Rivista)

Editore di

• ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS (Editore)