Spread of oxidative damage and antioxidative response through cell layers of tobacco callus after UV-C treatment (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Spread of oxidative damage and antioxidative response through cell layers of tobacco callus after UV-C treatment (Articolo in rivista) (literal)

Anno

• 2004-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/j.plaphy.2004.03.007 (literal)

Alternative label

• Massimo Zacchini1, Marina de Agazio1 (2004)
  Spread of oxidative damage and antioxidative response through cell layers of tobacco callus after UV-C treatment
  in Plant physiology and biochemistry (Paris)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Massimo Zacchini1, Marina de Agazio1 (literal)

Pagina inizio

• 445 (literal)

Pagina fine

• 450 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 42 (literal)

Rivista

• Plant physiology and biochemistry (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• 1Istituto di Biologia Agroambientale e Forestale, Consiglio Nazionale delle Ricerche, Via Salaria km 29.300, 00016 Monterotondo Scalo (Roma), Italy (literal)

Titolo

• Spread of oxidative damage and antioxidative response through cell layers of tobacco callus after UV-C treatment (literal)

Abstract

• Tobacco (Nicotiana tabacum L. cv. Petit Havana) callus cultures were exposed to UV-C high dose pulse-treatment (254 nm, 50 kJ m-2, 1 h-treatment). After 6, 24 and 48 h from the end of the treatment, calli were cut transversally in two layers and oxidative damage (malondialdehyde [MDA] and hydrogen peroxide), non-enzymatic (radical scavenging antioxidants [RSA] and polyamines) and enzymatic antioxidants (ascorbate peroxidase [APX, EC 1.11.1.11], glutathione reductase [GR, EC 1.6.4.2], catalase [CAT, EC 1.11.1.6] and guaiacol peroxidase [GPX, EC 1.11.1.7]) were evaluated.At each time-point data referred toUV-C treated calli were compared to data of untreated ones (control). Despite of a strong increase of H2O2 content, a slight cellular damage was observed in both upper and lower layers 24 and 48 h after UV-C treatment. An activation first of non-enzymatic antioxidants and then of enzymatic antioxidants was detected in UV-C treated calli. In particular, RSA and putrescine (PUT) accumulated 6 h after UV-C treatment while APX, GR and GPX enzyme activities increased 24 h after UV-C irradiation. Catalase activity did not change. UV-C-induced oxidative stress and antioxidative response were observed also in cell layers not directly exposed to UV irradiation, indicating that a stress signal was transmitted to the whole mass of callus. (literal)

Prodotto di

• Ripristino ecologico, biorimedio e biomitigazione (TA.P07.010.001) (Modulo)
• Istituto di biologia agro-ambientale e forestale (IBAF) (Istituto)

Autore CNR

• MASSIMO ZACCHINI (Unità di personale interno)
• MARINA DE AGAZIO (Unità di personale interno)

Insieme di parole chiave

• Keywords of "Spread of oxidative damage and antioxidative response through cell layers of tobacco callus after UV-C treatment" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• MASSIMO ZACCHINI (Unità di personale interno)
• MARINA DE AGAZIO (Unità di personale interno)

Prodotto

• Istituto di biologia agro-ambientale e forestale (IBAF) (Istituto)
• Ripristino ecologico, biorimedio e biomitigazione (TA.P07.010.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Plant physiology and biochemistry (Rivista)

Insieme di parole chiave di

• Keywords of "Spread of oxidative damage and antioxidative response through cell layers of tobacco callus after UV-C treatment" (Insieme di parole chiave)