Synergetic chemiluminescence and label-free dual detection for developing a hepatitis protein array (Articolo in rivista)

Type
Label
  • Synergetic chemiluminescence and label-free dual detection for developing a hepatitis protein array (Articolo in rivista) (literal)
Anno
  • 2011-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1016/j.jim.2011.06.004 (literal)
Alternative label
  • Pereira, C.; Yalcin, A.; Cretich, M.; Chiari, M.; Uenlue, M. S.; Nunes, D.; Bergstein, D. A. (2011)
    Synergetic chemiluminescence and label-free dual detection for developing a hepatitis protein array
    in Journal of immunological methods (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Pereira, C.; Yalcin, A.; Cretich, M.; Chiari, M.; Uenlue, M. S.; Nunes, D.; Bergstein, D. A. (literal)
Pagina inizio
  • 159 (literal)
Pagina fine
  • 164 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 371 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
  • 6 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 1-2 (literal)
Note
  • ISI Web of Science (WOS) (literal)
  • ISI Web of Science (WoS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Zoiray Technol Inc; Boston University; Consiglio Nazionale delle Ricerche (CNR); Boston Medical Center (literal)
Titolo
  • Synergetic chemiluminescence and label-free dual detection for developing a hepatitis protein array (literal)
Abstract
  • A dual detection system for protein arrays is presented that combines label-free detection by optical interference with chemiluminescence. A planar protein array that targets hepatitis B surface antigen is developed. Surface densities for individual antibody spots are quantitated using optical interference prior to use. Target binding (10 ng/ml) is detected label-free. Target binding (1 ng/ml) is detected by both optical interference and chemiluminescence with the inclusion of secondary antibodies. Binding results using both methods are found to be directly proportion to the capture probe density measured initially. The dual detection system provides the analytical utility of optical interference detection with the established clinical utility of chemiluminescence detection. (C) 2011 Elsevier B.V. All rights reserved. (literal)
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