An assay for the detection of grapevine leafroll-associated virus 3 using a single-chain fragment variable antibody (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• An assay for the detection of grapevine leafroll-associated virus 3 using a single-chain fragment variable antibody (Articolo in rivista) (literal)

Anno

• 2009-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1007/s00705-008-0263-y (literal)

Alternative label

• Cogotzi L; Giampetruzzi A; Nölke G; Orecchia M; Elicio V; Castellano MA; Martelli GP; Fischer R; Schillberg S; Saldarelli P (2009)
  An assay for the detection of grapevine leafroll-associated virus 3 using a single-chain fragment variable antibody
  in Archives of virology
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Cogotzi L; Giampetruzzi A; Nölke G; Orecchia M; Elicio V; Castellano MA; Martelli GP; Fischer R; Schillberg S; Saldarelli P (literal)

Pagina inizio

• 19 (literal)

Pagina fine

• 26 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 154 (literal)

Rivista

• Archives of virology (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 8 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Department of Plant Protection and Applied Microbiology, University of Bari, Via Amendola 165/A, 70126 Bari, Italy Institute for Molecular Biotechnology (Biology VII), RWTH Aachen, Worringerweg 1, 52074 Aachen, Germany GlaxoSmithKline, Gunnels Wood Road, Stevenage, Herts SG1 2NY, UK Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Forckenbeckstrasse 6, 52074 Aachen, Germany Agritest s.r.l, Valenzano, Bari, Italy (literal)

Titolo

• An assay for the detection of grapevine leafroll-associated virus 3 using a single-chain fragment variable antibody (literal)

Abstract

• Grapevine leafroll-associated virus 3 (GLRaV- 3) is a major pathogen of grapevine. A previously described single-chain fragment variable (scFv) antibody (scFvLR3), directed against the coat protein (CP) of GLRaV-3, was expressed in Escherichia coli and used to develop a diagnostic ELISA kit. The antibody was fused to the light chain constant domain of human immunoglobulin to create the bivalent reagent CL-LR3, which was purified from the periplasmic fraction, giving a yield of *5 mg/l. The sensitivity of the reagent against recombinant GLRaV-3 CP was 0.1 ng. The sensitivity, specificity and durability of the reagent was similar to a commercial kit. TheCL-LR3 showed a weak cross-reaction in immune electron microscopy assays to GLRaV-1 and -7, but not with the phylogenetically more distant GLRaV-2. A fully recombinant kit was developed with the inclusion of a recombinant GLRaV-3 CP expressed in bacteria, thus avoiding problems associated with virus propagation and purification. This system represents a rapid, simple, sensitive and standardized diagnostic protocol for GLRaV-3 detection. (literal)

Prodotto di

• Interazioni biologiche e molecolari delle piante con virus e agenti patogeni virus-simili (AG.P01.010.001) (Modulo)

Autore CNR

• PASQUALE SALDARELLI (Unità di personale interno)

Incoming links:

Autore CNR di

• PASQUALE SALDARELLI (Unità di personale interno)

Prodotto

• Interazioni biologiche e molecolari delle piante con virus e agenti patogeni virus-simili (AG.P01.010.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Archives of virology (Rivista)