Purification and characterization of thermostable xylanase and b-xylosidase by the thermophilic bacterium Bacillus thermantarcticus. (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Purification and characterization of thermostable xylanase and b-xylosidase by the thermophilic bacterium Bacillus thermantarcticus. (Articolo in rivista) (literal)

Anno

• 2004-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/j.resmic.2004.02.001 (literal)

Alternative label

• L. Lama; V. Calandrelli; A. Gambacorta; B. Nicolaus (2004)
  Purification and characterization of thermostable xylanase and b-xylosidase by the thermophilic bacterium Bacillus thermantarcticus.
  in Research in microbiology (Paris)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• L. Lama; V. Calandrelli; A. Gambacorta; B. Nicolaus (literal)

Pagina inizio

• 283 (literal)

Pagina fine

• 289 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 155 (literal)

Rivista

• Research in microbiology (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 4 (literal)

Note

• ISI Web of Science (WOS) (literal)
• Scopu (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Istituto di Chimica Biomolecolare, CNR, Via Campi Flegrei, 34, 80078 Pozzuoli, Napoli, Italy (literal)

Titolo

• Purification and characterization of thermostable xylanase and b-xylosidase by the thermophilic bacterium Bacillus thermantarcticus. (literal)

Abstract

• Bacillus thermantarcticus, a thermophilic bacterium isolated from Antarctic geothermal soil near the crater ofMount Melbourne, produced extracellular xylanase (1,4-b-D-xylan xylanohydrolase; E.C. 3.2.1.8) and b-xylosidase (1,4-b-D-xylan xylohydrolase; E.C. 3.2.1.37). Each extracellular enzyme was separated by gel filtration with Sephacryl S-200 and further purified to homogeneity (119-fold for xylanase and 160- fold for b-xylosidase). The optimum temperatures were 80 oC for xylanase at pH 5.6 and 70 oC for b-xylosidase at pH 6.0. The isoelectric points and molecular masses were 4.8 and 45 kDa for xylanase and 4.2 and 150 kDa for b-xylosidase, respectively. Xylanase was stable at 60 oC for 24 h, whereas it showed a half life at 70 oC of 24 h and at 80oC for 50 min. b-xylosidase activity did not decrease after 1 h at 60oC. Km of xylanase for xylan was 1.6 mg/ml, Km of b-xylosidase for p-nitrophenyl-b-D-xylopyranoside was 0.5 mM and for o-nitrophenyl-b-D-xylopyranoside was 1.28 mM. The action of two enzymes on xylan gave only xylose. (literal)

Prodotto di

• LAMA - Gli Estremofili: extremozimi, esopolisaccaridi e loro applicazioni (PM.P01.008.001) (Modulo)
• Istituto di chimica biomolecolare (ICB) (Istituto)

Autore CNR

• BARBARA NICOLAUS (Unità di personale interno)
• VALERIA CALANDRELLI (Persona)
• AGATA GAMBACORTA (Unità di personale interno)
• LICIA LAMA (Persona)

Insieme di parole chiave

• Parole chiave di "Purification and characterization of thermostable xylanase and b-xylosidase by the thermophilic bacterium Bacillus thermantarcticus." (Insieme di parole chiave)

Incoming links:

Autore CNR di

• LICIA LAMA (Persona)
• VALERIA CALANDRELLI (Persona)
• BARBARA NICOLAUS (Unità di personale interno)
• AGATA GAMBACORTA (Unità di personale interno)

Prodotto

• Istituto di chimica biomolecolare (ICB) (Istituto)
• LAMA - Gli Estremofili: extremozimi, esopolisaccaridi e loro applicazioni (PM.P01.008.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Research in microbiology (Rivista)

Insieme di parole chiave di

• Parole chiave di "Purification and characterization of thermostable xylanase and b-xylosidase by the thermophilic bacterium Bacillus thermantarcticus." (Insieme di parole chiave)