Temporal dynamics of 1H-NMR visible metabolites during radiation-induced apoptosis in MG-63 human osteosarcoma spheroids (Articolo in rivista)

Type
Label
  • Temporal dynamics of 1H-NMR visible metabolites during radiation-induced apoptosis in MG-63 human osteosarcoma spheroids (Articolo in rivista) (literal)
Anno
  • 2006-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1667/RR0635.1 (literal)
Alternative label
  • Santini, M. T.; Romano, R.; Rainaldi, G.; Indovina, P.; Ferrante, A.; Motta, A.; Indovina, P. L.; (2006)
    Temporal dynamics of 1H-NMR visible metabolites during radiation-induced apoptosis in MG-63 human osteosarcoma spheroids
    in Radiation research
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Santini, M. T.; Romano, R.; Rainaldi, G.; Indovina, P.; Ferrante, A.; Motta, A.; Indovina, P. L.; (literal)
Pagina inizio
  • 734 (literal)
Pagina fine
  • 745 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 166 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
  • Scopu (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Maria Teresa Santini a,b, Rocco Romano c, Gabriella Rainaldi a,b, Paola Indovina a, Antonella Ferrante a, Andrea Motta d, Pietro Luigi Indovina b,e, a Dipartimento di Ematologia, Oncologia e Medicina Molecolare, Istituto Superiore di Sanità, 00161, Rome, Italy; b CNR-INFM, Unità di Napoli, Complesso Universitario Monte S. Angelo, 80126, Naples, Italy; c Dipartimento di Scienze Farmaceutiche, Università degli Studi di Salerno, 84084, Fisciano, Salerno, Italy; d Consiglio Nazionale delle Ricerche, Istituto di Chimica Biomolecolare, Comprensorio A. Olivetti, Edificio 70, 80078 Pozzuoli, Naples, Italy; e Dipartimento di Scienze Fisiche, Università di Napoli ''Federico II'', Complesso Universitario Monte S. Angelo, 80126, Naples, Italy. (literal)
Titolo
  • Temporal dynamics of 1H-NMR visible metabolites during radiation-induced apoptosis in MG-63 human osteosarcoma spheroids (literal)
Abstract
  • The metabolic changes that occur as a function of time in MG-63 osteosarcoma three-dimensional tumor spheroids undergoing radiation-induced apoptosis were studied using high-resolution proton nuclear magnetic resonance (1HNMR) spectroscopy. Specifically, the 1H-NMR spectra of MG-63 spheroids collected at 24, 48 and 72 h after exposure to 5 Gy of ionizing radiation were compared to the spectra of their respective controls. Small spheroids (about 50-80 um in diameter) with no hypoxic center were used. Apoptosis was verified by both staining of spheroid DNA with the Hoechst 33258 dye and determination of caspase 3 enzyme activity at the three times examined. The results demonstrate that, as the percentage of apoptosis rises with time after exposure to ionizing radiation, the metabolic changes that take place in MG-63 spheroids follow very precise temporal dynamics. In particular, significant time-related increases in both CH2 and CH3 mobile lipids, considered by many authors as markers of apoptosis, were observed. In addition, temporal variations were also observed in choline-containing metabolites, reduced glutathione (GSH), glutamine/glutamate, taurine, alanine, creatine/phosphocreatine and lactate. These data show that in addition to CH2 and CH3 lipids, other metabolites can also be extremely useful in a deeper understanding of the temporal dynamics of radiation-induced apoptosis. This comprehension is particularly important in spheroids, a cell model of great complexity that resembles in vivo tumors much more closely than monolayer cultures. Ultimately, it is hoped that such studies can help to evaluate the outcome of radiotherapy protocols more accurately. (literal)
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