http://www.cnr.it/ontology/cnr/individuo/prodotto/ID17582
Functionalization of poly(dimethylsiloxane) by chemisorption of copolymers: DNA microarrays for pathogen detection (Articolo in rivista)
- Type
- Label
- Functionalization of poly(dimethylsiloxane) by chemisorption of copolymers: DNA microarrays for pathogen detection (Articolo in rivista) (literal)
- Anno
- 2008-01-01T00:00:00+01:00 (literal)
- Alternative label
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Cretich M.; Sedini V.; Damin F.; Di Carlo G.; Oldani C.; Chiari M. (literal)
- Pagina inizio
- Pagina fine
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
- Rivista
- Note
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Istituto di Chimica del Riconoscimento Molecolare (ICRM), CNR (literal)
- Titolo
- Functionalization of poly(dimethylsiloxane) by chemisorption of copolymers: DNA microarrays for pathogen detection (literal)
- Abstract
- A robust method for coating polydimethylsiloxane (PDMS) allowing covalent binding of biomolecules is reported. The coating is based on adsorption of a copolymer, copoly(DMA-NAS-MAPS), bearing functional groups. The polymer is synthesized by free radical polymerization; it self-adsorbs onto the PDMS very rapidly, typically in 30 min, even without any PDMS surface pre-treatment. PDMS slides coated by copoly(DMA-NAS-MAPS) covalently bind amino-modified DNA fragments and have been tested in bacterial genotyping experiments. The usefulness of this functional coating on PDMS structures in lab-on-chip devices powered by microfluidics has been demonstrated. The copoly(DMA-NAS-MAPS) coated PDMS slides were used as flexible components of a microcell in semi-automated DNA microarray experiments for rapid food pathogen identification. (literal)
- Prodotto di
- Autore CNR
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