http://www.cnr.it/ontology/cnr/individuo/prodotto/ID17567
Enantiomeric separation of baclofen by capillary electrophoresis tandem mass spectrometry with sulfobutylether-beta-cyclodextrin as chiral selector in partial filling mode (Articolo in rivista)
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- Label
- Enantiomeric separation of baclofen by capillary electrophoresis tandem mass spectrometry with sulfobutylether-beta-cyclodextrin as chiral selector in partial filling mode (Articolo in rivista) (literal)
- Anno
- 2008-01-01T00:00:00+01:00 (literal)
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- Desiderio C. ; Rossetti D.V.; Perri F.; Giardina B.; Messana I.; Castagnola M. (literal)
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- corresponding author: DESIDERIO Claudia (literal)
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- ISI Web of Science (WOS) (literal)
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- Desiderio C. (a), Rossetti D.V. (b), Perri F.(b), Giardina B.(a,b), Messana I.(c), Castagnola M.(a,b)
(a) Istituto di Chimica del Riconoscimento Molecolare, Consiglio Nazionale delle Ricerche, L.go F. Vito 1, 00168 Roma, Italy
(b) Istituto di Biochimica e Biochimica Clinica, Università Cattolica del Sacro Cuore, L.go F. Vito 1, 00168 Roma, Italy
(c) Dipartimento di Scienze Applicate ai Biosistemi, Università di Cagliari, Campus Monserrato, 09042 Monserrato (Ca), Italy (literal)
- Titolo
- Enantiomeric separation of baclofen by capillary electrophoresis tandem mass spectrometry with sulfobutylether-beta-cyclodextrin as chiral selector in partial filling mode (literal)
- Abstract
- Capillary electrophoresis (CE) coupled to tandem mass spectrometry was applied to the chiral separation
of baclofen using sulfobutylether-?-cyclodextrin chiral selector in partial filling counter current mode.
On-line UV detection was simultaneously used. Method optimization was performed by studying the
effect of cyclodextrin and BGE concentration as well as sheath liquid composition on analyte migration
time and enantiomeric resolution. The cyclodextrin showed stereoselective complexation towards
baclofen enantiomers, allowing chiral resolution at low concentration. The CE capillary protrusion from
the ESI needle relevantly affected the chiral resolution and the analyte migration time. Complete enantiomeric
separation was obtained by using 0.25M formic acid BGE containing 1.75mM of chiral selector
andwater/methanol (30:70, v/v) 3% formic acid as sheath liquid. The method exhibited a LOD of 0.1?g/mL
(racemic concentration) in MS3 product ion scan mode of detection and was applied to the analysis of
racemic baclofen in pharmaceutical formulations. (literal)
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